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Anticorps Western blot

Les anticorps pour Western blot (WB) sont optimisés pour la détection de protéines dénaturées et validés pour produire des bandes claires et spécifiques à la masse moléculaire attendue. Utilisez les filtres ci-dessous pour identifier rapidement des anticorps primaires adaptés à votre cible, à votre espèce et à vos exigences expérimentales.

En savoir plus sur le Western blot

Validation des anticorps Western blot

Les anticorps utilisés pour le Western blot doivent détecter de manière fiable les protéines cibles dans des conditions dénaturantes. Les produits de cette catégorie sont validés pour les applications WB et sélectionnés pour la détection spécifique de bandes à la masse moléculaire attendue. Lorsque disponibles, les données de validation incluent des images expérimentales, des tests indépendants et des références bibliographiques.

Considérations pour le Western blot

La dénaturation des protéines lors du Western blot peut modifier la structure des épitopes, rendant le choix des anticorps crucial. Les anticorps validés pour le WB sont optimisés pour reconnaître des épitopes linéarisés et réduire les liaisons non spécifiques.

Les anticorps monoclonaux offrent une grande spécificité et des résultats reproductibles, tandis que les anticorps polyclonaux peuvent fournir un signal plus fort grâce à la reconnaissance de multiples épitopes. Les anticorps recombinants combinent spécificité définie et reproductibilité améliorée.

Western blot : principes, protocoles et dépannage

En savoir plus sur le Western blot, y compris les protocoles et le dépannage

Utilisez les filtres ci-dessous pour affiner votre recherche d’anticorps Western blot selon la cible, l’espèce et les exigences expérimentales.

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516.987 Résultats

Iba1 anticorps (C-Term)

IBA1 Reactivité: Humain WB, IHC, ICC, IF, FACS, IHC (p), IHC (fro), IHC (ff) Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody (ABIN2857032)

IHC-P Image Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.

Neuropathology in the central nervous system of Fuca1-deficient mice.

18 images

15 références

1 validation

N° du produit ABIN2857032

583,17 €

Plus frais de livraison 40,00 € et TVA

100 μL

Destination: France

Envoi sous 7 à 9 jours ouvrables

Détails

Independently Validated

GFP anticorps

GFP Reactivité: Aequorea victoria WB, ELISA, FM Hôte: Chèvre Polyclonal unconjugated

MSCs are traced in special organized regions.

IHC

Immunofluorescence Microscopy of GFP-GOAT-Antibody.

Western Blot of Rabbit anti-GFP antibody.

Immunofluorescence Microscopy of Anti-GFP (GOAT) Antibody.

Western Blot of Goat anti-GFP antibody with lysates.

Western Blot of Goat anti-GFP Antibody with Serums.

Tyrosine phosphorylation of HIPK mutants.

Maximal activity of HIPKs depends on the activation loop tyrosine.

Subcellular distribution of HIPK mutants.

15 images

178 références

N° du produit ABIN100085

350,00 €

Plus frais de livraison 40,00 € et TVA

100 μL (Autres quantités disponibles)

Destination: France

Envoi sous 2 à 3 jours ouvrables

Détails

GAPDH anticorps

GAPDH Reactivité: Humain WB, IF, IP, ICC, IHC (p) Hôte: Lapin Polyclonal unconjugated

WB Image GAPDH antibody detects GAPDH protein by western blot analysis.

IHC-P Image GAPDH antibody detects GAPDH protein at cytoplasm in mouse testis by immunohistochemical analysis.

IHC

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH, antibody (green) at 1:500 dilution.

IP Image Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody, Western blot analysis was performed using GAPDH antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image Immunohistochemical analysis of human salivary gland cancer, using GAPDH, antibody at 1:500 dilution.

IHC

IHC-P Image GAPDH antibody detects GAPDH protein at cytoplasm in rat heart by immunohistochemical analysis.

IHC

Immunoblotting of LEG-1 from the treated fibroblasts and the measurement of ROS within the treated fibroblasts.

Effects of quercetin on the inflammatory response in H2O2-treated H9C2 cells.

Effects of quercetin on cell apoptosis in H2O2-treated H9C2 cells.

Comprehensive immunofluorescence images and immunoblotting analysis of the differentially expressed proteins identified by MALDI-TOF MS.

Gene trap screen for mitochondrial phenotypes identifies Sucla2 mutant allele.

Sucla2 mutant embryos display growth deficiency and placental mtDNA depletion.

Sucla2-deficient embryo tissues exhibit progressive, functionally significant mtDNA depletion and elevated MMA.

Effects of quercetin on doxorubicin-induced changes of cell viability, cell apoptosis and cell morphology in H9C2 cells.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

RAGE expression in human peripheral nerve fibers.

Arecoline induces the transdifferentiation of buccal mucosal fibroblasts (BMFs) into myofibroblasts.

Arecoline-induced α-smooth muscle actin (α-SMA) expression is mediated by the direct binding of zinc finger E-box binding homeobox 1 (ZEB1) to the α-SMA promoter.

Long-term exposure of buccal mucosal fibroblasts (BMFs) to arecoline induces fibrogenic gene expression and collagen contraction.

Arecoline-induced zinc finger E-box binding homeobox 1 (ZEB1) expression is independent of reactive oxygen species, but is mediated by the activation of insulin-like growth factor receptor-1 (IGF-1R).

Regulation of NED by REST in LNCaP cells.

IL-6 induces NED in LNCaP cells and this is concomitant with increased autophagy.

Knockdown of beclin1 suppresses IL-6 induced NED under androgen deprivation conditions.

Knockdown of Atg5 suppresses IL-6 induced NED under androgen deprivation conditions.

IL-6 treatment inhibits mTOR via the activation of AMPK pathway.

Modulation of curcumin in oxidative stress-regulating genes.

Arecoline decreased membrane expression of ZO-1 in confluent TM4 cells.

Arecoline activates ERK1/2 MAPKs and increases TNF-alpha production.

Activation of ERK1/2 MAPKs is important for TNF-alpha induction and ZO-1 protein redistribution by arecoline in TM4 cells.

DZNep-mediated sensitization to TRAIL correlates with enhanced activation of caspase signaling and involvement of the mitochondrial apoptosis pathway.

Early and accelerated processing of caspase-8 with DZNep treatment in lymphoma cell lines.

DZNep-mediated enhanced apoptosis correlates with reduced cFLIP expression levels.

Effects of different proteins on post-treatment lipolysis markers.

Creation and characterization of Supt4h knockout mice.

Western blot analysis of FOXM1 and potential target expression in GEP-NEN cell linesAll cell lines including the non-neuroendocrine HT-29 and the FOXM1 overexpressing U2OS control cell lines expressed FOXM1 A.

The expression of BH3-only protein, pro-apoptotic proteins and anti-apoptotic proteins in LNCaP or PC3 cells after treatment with paclitaxel.

The effect of ABT-199, ABT-263 or ABT-263 in combination with paclitaxel on LNCaP or PC3 cells.

NS5 with mutation on residue 19 (M19A) showed reduced binding ability with MTP.

Diverse fuels promote TNKS autoPARsylation and turnover in INS-1 cells.

Promoter hypermethylation of sGCβ1 in MCF-7 and sGCα1 in MDA-MB-231 cells.

Over-expression of sGCα1 and sGCβ1 in MDA-MB-231 cells affected cGMP production, proliferation, survival, and cell cycle.

Over-expression of sGC induced apoptosis and cell cycle arrest.

GSK3β inhibitor SB415286 inhibits the HYS-32-induced phosphorylation of GSK3β-pY216 and attenuates the HYS-32-induced microtubule catastrophes.

Caspase-dependent apoptotic pathways were seen in LNCaP, but not in PC3 cells, in response to G2/M arrest.

LY294002 inhibits the HYS-32-induced phosphorylation of GSK3β-pS9 and GSK3β-pY216.

Overexpression of miR-29a in the murine model resulted in downregulation of fibrosis and HDAC4 in the liver of mice after BDL.

Effects of TLCA on HDAC4 expression in cultured primary HSCs.

Overexpression of miR-29a decreased HDAC4 expression in primary HSCs.

Overexpression of miR-29a increased histone H3 at lysine 9 (H3K9Ac) and decreased Smad3 expression in HSCs.

Western blot analysis shows pErk reduction through selumetinib treatment and by removal of stromal support.

Phospho-flow measures long-term inhibition by selumetinib of pErk generated in BCP-ALL cells by extracellular serum stimulation.

Diagnosis ALL samples differ in ability to activate Erk1/2 upon serum stimulation.

Mek pathway inhibition in combination-treated BCP-ALL cells by phospho-flow and Western blot.

Demyelination/remyelination and decreased P0 expression in dKO mice.

Loss of neurofascins and Caspr in dKO mice.

Inhibition of ERK1/2 inhibits ETO-induced centrosome amplification.

Chloroquine inhibits DNA damage-induced centrosome amplification.

Chloroquine inhibits ETO-activated CDK2 and ERK1/2 in adrenocortical Y1 tumor cells.

HSPs positively regulate IE2 transaction activity.

Comparison of the mRNA levels of Smad7 (a) and protein levels of Smad3 (b) expressions in the liver of WT and miR-29Tg mice following bile duct ligation (BDL).

Overexpression of miR-29a in the murine model caused the upregulation of β-catenin (a,b), and Dkk1 (d) as well as the downregulation of phospho-β-catenin (c) and GSK3β (e) in the liver of mice following bile duct ligation.

SFN suppressed HCV protein expression and RNA replication.

SFN-mediated HO-1 induction in HCV replicon cells.

SFN inhibited HCV replication through HO-1 dependent pathway.

SFN induced bilirubin production in HCV replicon cells.

SFN inhibited HCV replication by upregulating Nrf2 expression.

SFN-mediated Nrf2/HO-1 activation was PI3K dependent.

Hypoxia induces NED of LNCaP cells concomitant with down-regulation REST protein levels but not REST mRNA(A) LNCaP cells were treated with hypoxia (2 % O2) for 3 days.

Inhibition of neurite elongation by REST overexpression(A) LNCaP-TR-REST cells were treated with 0.

AMPK/mTOR pathway is activated by hypoxia treatment and REST knockdown(A) LNCaP cells were treated with normoxia (N) or hypoxia (H) (2 % O2) for 3 days (left panel).

Hypoxia-induced autophagy activation of LNCaP cells(A) LNCaP-eGFP-LC3 cells were treated with hypoxia (2 % O2) for 3 days.

Chemical inhibition of autophagy flux by chloroquine (CQ) suppresses hypoxia-induced NED in LNCaP cells(A) LNCaP cells were treated with hypoxia (2 % O2) in the absence or presence of 50 μM CQ for 3 days.

Knockdown of beclin 1 suppresses hypoxia-induced NED(A) LNCaP-TR-shBeclin1 cells were treated with 1 μg/mL Dox to induce beclin 1 knockdown for 4 days under normoxia and hypoxia (2 % O2).

Disruption of autophagy by treatment with CiQ derivativesA.

Induction of apoptosis by treatment with CiQ derivativesA.

Induction of lysosome dysfunction by treatment with CiQ derivativesA and B.

Differential expression of CDK1 pTyr15 in colorectal cancer cells and tissues.

Senataxin is SUMOylated at the XY chromosomes in spermatocytes.

120 images

105 références

N° du produit ABIN2857072

583,17 €

Plus frais de livraison 40,00 € et TVA

100 μL

Destination: France

Envoi sous 7 à 9 jours ouvrables

Détails

TARDBP anticorps (AA 1-260)

TARDBP Reactivité: Humain WB, IF, ELISA, IP, IHC (p), RNAi Hôte: Souris Monoclonal 2E2-D3 unconjugated

Western Blot detection against Immunogen (54.

Immunoperoxidase of monoclonal antibody to TARDBP on formalin-fixed paraffin-embedded human leiomyosarcoma tissue [antibody concentration 3 ug/ml]

ISt

Immunofluorescence of monoclonal antibody to TARDBP on HeLa cell.

TARDBP monoclonal antibody (M01), clone 2E2-D3 Western Blot analysis of TARDBP expression in A-431 .

Western blot analysis of TARDBP over-expressed 293 cell line, cotransfected with TARDBP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1).

Western Blot analysis of TARDBP expression in transfected 293T cell line by TARDBP monoclonal antibody (M01), clone 2E2-D3.

Immunoprecipitation of TARDBP transfected lysate using anti-TARDBP monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with TARDBP MaxPab rabbit polyclonal antibody.

Detection limit for recombinant GST tagged TARDBP is 0.

ELISA

8 images

122 références

N° du produit ABIN565080

651,20 €

Plus frais de livraison 40,00 € et TVA

100 μg

Destination: France

Envoi sous 8 à 9 jours ouvrables

Détails

beta Amyloid anticorps (C-Term)

Abeta Reactivité: Humain, Souris, Rat WB, ELISA, IF Hôte: Souris Monoclonal 9E8-1A3 unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (Abeta) (C-Term) antibody (ABIN5508784)

IHC

Western blots of the monoclonal antibody binding to different amyloid beta (Abeta) regions of human and mouse protein, using 12% A/B (acrylamide/bisacrylamide) or 10% Tricine matrix.

Fluorescent microscopy image (appotome) of 6 months-old APP/PS1 mouse hypocampus and somatosensory cortex.

Immunofluorescence slices with the anti-amyloid beta antibody from different mouse genotypes.

Western blots of monoclonal antibody reactivity with brain extracts from 6 to 15 month-old transgenic (tg) APP/PS1 and wild-type (wt) mice.

6 images

4 références

2 validations

N° du produit ABIN5508784

298,10 €

Plus frais de livraison 40,00 € et TVA

100 μg

Destination: France

Envoi sous 3 à 4 jours ouvrables

Détails

Independently Validated

LEF1 anticorps (AA 100-399)

LEF1 Reactivité: Humain WB Hôte: Lapin Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 100-399) antibody (ABIN1680678)

CUT&RUN

Western blot analysis of extracts of various cell lines, using LEF1 antibody (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021) at 1:1000 dilution.

Immunofluorescence analysis of rat thymus cells using LEF1 Rabbit pAb (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021) at dilution of 1:25 (40x lens).

CUT&RUN

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

CUT&RUN

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg LEF1 antibody (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021).

Immunofluorescence analysis of mouse thymus cells using LEF1 Rabbit pAb (ABIN1680678, ABIN5663809, ABIN5663811 and ABIN6214021) at dilution of 1:25 (40x lens).

8 images

2 références

1 validation

N° du produit ABIN1680678

320,00 €

Plus frais de livraison 40,00 € et TVA

100 μg (Autres quantités disponibles)

Destination: France

Envoi sous 8 à 10 jours ouvrables

Détails

Independently Validated

NFKB1 anticorps (AA 740-964)

NFKB1 Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 740-964) antibody (ABIN6144571)

Western blot analysis of extracts of various cell lines, using NFKB1 antibody.

Immunofluorescence analysis of U2OS cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using NFKB1 antibody.

IHC (p)

Immunofluorescence analysis of A549 cells using NFKB1 antibody.

Immunofluorescence analysis of A-549 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

IHC

Immunofluorescence analysis of NIH/3T3 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

8 images

2 références

1 validation

N° du produit ABIN6144571

320,00 €

Plus frais de livraison 40,00 € et TVA

100 μL (Autres quantités disponibles)

Destination: France

Envoi sous 8 à 10 jours ouvrables

Détails

Independently Validated

Complement Factor H anticorps (AA 20-270)

CFH Reactivité: Humain WB, IHC, IF Hôte: Lapin Polyclonal unconjugated

Immunofluorescence validation image for anti-Complement Factor H (CFH) (AA 20-270) antibody (ABIN3023097)

Western blot analysis of extracts of HepG2 cell line, using CFH antibody.

Immunohistochemistry of paraffin-embedded human placenta using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

IHC

Western blot analysis of extracts of Rat liver, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Western blot analysis of extracts of Human plasma, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Western blot analysis of extracts of various cell lines, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human liver cancer using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

IHC

Immunofluorescence analysis of HepG2 cells using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

8 images

2 références

1 validation

N° du produit ABIN3023097

320,00 €

Plus frais de livraison 40,00 € et TVA

100 μL (Autres quantités disponibles)

Destination: France

Envoi sous 8 à 10 jours ouvrables

Détails

Independently Validated

CD200 anticorps (Internal Region)

CD200 Reactivité: Humain WB, ELISA, IHC (p) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-CD200 (CD200) (Internal Region) antibody (ABIN3187966)

IHC

Western Blotting (WB) image for anti-CD200 (CD200) (Internal Region) antibody (ABIN3187966)

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.

IHC

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver, antibody was diluted at 1:100.

IHC

6 images

1 reference

1 validation

N° du produit ABIN3187966

377,51 €

Plus frais de livraison 40,00 € et TVA

100 μL (Autres quantités disponibles)

Destination: France

Envoi sous 8 à 10 jours ouvrables

Détails

Independently Validated

MAP2K5 anticorps (AA 251-350)

MAP2K5 Reactivité: Humain, Souris, Rat WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 251-350) antibody (ABIN754183)

IHC

Mouse heart lysates probed with Rabbit Anti-MEK5 Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Independently Validated Antibody, image provided by Science Direct, badge number 029644:Formalin-fixed and paraffin embedded human breast myoepithelial and glandular tissue labeled with Anti-MEK5 Polyclonal Antibody, Unconjugated (ABIN754183) at 1:250 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-MEK5/MAP2K5 Polyclonal Antibody, Unconjugated (ABIN754183) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

7 images

2 références

1 validation

N° du produit ABIN754183

357,70 €

Plus frais de livraison 40,00 € et TVA

100 μL

Destination: France

Envoi sous 8 à 11 jours ouvrables

Détails

Independently Validated

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