Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Humain/Mouse MAPK Phosphorylation Array

Reactivité: Humain, Souris AA Chemiluminescent Semi-Quantitative Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Membrane
N° du produit ABIN4955901
  • Reactivité
    Humain, Souris
    Méthode de détection
    Chemiluminescent
    Type de méthode
    Sandwich ELISA
    Application
    Antibody Array (AA)
    Fonction
    C-Series Human/Mouse MAPK Phosphorylation Antibody Array 1 Kit. Detects 17 Human/mouse proteins. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum, Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    Akt (P-Ser473), CREB (P-Ser133), ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187), GSK3a (P-Ser21), GSK3b (P-Ser9), HSP27 (P-Ser82), JNK (P-Thr183), MEK (P-Ser217/Ser221), MKK3 (P-Ser189), MKK6 (P-Ser207), MSK2 (P-Ser360), mTOR (P-Ser2448), p38 (P-Thr180/Tyr182), P53 (P-Ser15), P70S6K (P-Thr421/Ser424), RSK1 (P-Ser380), RSK2 (P-Ser386)
    Attributs du produit
    • Easy to use
    • No specialized equipment needed
    • Compatible with nearly any liquid sample
    • Proven technology (many publications)
    • Highly sensitive (pg/mL)
    • Sandwich ELISA specificity
    • Higher density than ELISA, Western blot or bead-based multiplex
    Ingrédients
    Antibody Array Membranes
    Biotinylated Detection Antibody Cocktail
    Blocking Buffer
    Wash Buffers 1 and 2
    Cell & Tissue Lysis Buffer
    Detection Buffers C and D
    Plastic Incubation Tray
    Protease Inhibitor Cocktail (in select kits)
    Matériel non inclus
    Pipettors, pipet tips and other common lab consumables
    Orbital shaker or oscillating rocker
    Tissue Paper, blotting paper or chromatography paper
    Adhesive tape or Saran Wrap
    Distilled or de-ionized water
    A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
  • Indications d'application
    Perform ALL incubation and wash steps under gentle rotation or rocking motion (~0.5 to 1 cycle/sec) using an orbital shaker or oscillating rocker to ensure complete and even reagent/sample coverage. Rocking/rotating too vigorously may cause foaming or bubbles to appear on the membrane surface which, should be avoided. All washes and incubations should be performed in the Incubation Tray (ITEM 10) provided in the kit. Cover the Incubation Tray with the lid provided during all incubation steps to avoid evaporation and outside debris contamination. Ensure the membranes are completely covered with sufficient sample or reagent volume during each incubation. Avoid forceful pipetting directly onto the membrane, instead, gently pipette samples and reagents into a corner of each well. Aspirate samples and reagents completely after each step by suctioning off excess liquid with a pipette. Tilting the tray so the liquid moves to a corner and then pipetting is an effective method. Optional overnight incubations may be performed for the following step to increase overall spot signal intensities:
    - Sample Incubation
    - Biotinylated Antibody Cocktail Incubation
    - HRP-Streptavidin Incubation
    Commentaires

    The C-Series arrays feature chemiluminescent signal detection. The antibodies are spotted on nitrocellulose membrane solid supports and are handled in a very similar manner to Western blots.
    All C-Series arrays work on the sandwich ELISA principle, utilizing a matched pair of antibodies: an immobilized capture antibody and a corresponding biotinylated detection antibody.

    Volume d'échantillon
    1 mL
    Plaque
    Membrane
    Protocole
    1. Block membranes
    2. Incubate with Sample
    3. Incubate with Biotinylated Detection Antibody Cocktail
    4. Incubate with HRP-Conjugated Streptavidin
    5. Incubate with Detection Buffers
    6. Image with chemiluminescent imaging system
    7. Perform densitometry and analysis
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The antibody printed side of each membrane is marked by a dash (-) or number (#) in the upper left corner. Do not allow membranes to dry out during the experiment or they may become fragile and break OR high and/or uneven background may occur. Grasp membranes by the corners or edges only using forceps. DO NOT touch printed antibody spots.
    Stock
    -20 °C
    Stockage commentaire
    For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  • Schuett, Schuett, Oberoi, Koch, Pretzer, Luchtefeld, Schieffer, Grote: "NADPH oxidase NOX2 mediates TLR2/6-dependent release of GM-CSF from endothelial cells." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 31, Issue 6, pp. 2612-2624, (2017) (PubMed).

Vous êtes ici:
Support technique