Ni-Charged Magnetic Beads

N° du produit ABIN400578

Aperçu rapide pour Ni-Charged Magnetic Beads (ABIN400578)

Application: Immunoprecipitation (IP)

Bead Ligand: Nickel ions

Bead Matrix: Magnetic particles

Bead Size: 40 µm

Détail du produit

Attributs du produit: Ni-charged MagBeads (8 mL 25% slurry) are average 40µm in size, superparamagnetic beads pre-charged with nickel ions and ready to use for quick and small-scale purification of His-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Ni-Charged MagBeads have a binding capacity of 5 to 20 mg 6×His-tagged protein per 1 mL settled beads (e.g. 4 mL 25% slurry).The beads are compatible with magnetic separation-based high throughput applications. Proteins can be purified under non-denaturing conditions or in the presence of 8 M urea.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 25% settled beads

Stock: 4 °C

Stockage commentaire: Store at 4°C, do NOT freeze.

Date de péremption: 12 months

Référence

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Dong, Tian, Gomez, Li: "Regulated proteolysis of the alternative sigma factor SigX in Streptococcus mutans: implication in the escape from competence." dans: BMC microbiology, Vol. 14, pp. 183, (2014) (PubMed).

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Wiltrout, Goodenbour, Fréchin, Pan: "Misacylation of tRNA with methionine in Saccharomyces cerevisiae." dans: Nucleic acids research, Vol. 40, Issue 20, pp. 10494-506, (2012) (PubMed).