ALFA Catcher ST

N° du produit ABIN7272858

Aperçu rapide pour ALFA Catcher ST (ABIN7272858)

Antigène: ALFA-Tag

Application: Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Protein Complex Immunoprecipitation (Co-IP)

Bead Ligand: Antibody

Bead Matrix: Agarose beads

Bead Size: 90 μm

Détail du produit

Fonction: ALFA Catcher ST (for “Super Tight”) is an innovative, high-affinity resin (Agarose) designed for efficient and clean pull-down of ALFA-tagged proteins.

Specificité: Recognizes the ALFA-tag (SRLEEELRRRLTE)

Attributs du produit: ALFA CatcherST is based on a single-domain antibody (sdAb), which is covalently immobilized on 4% cross-linked agarose or agarose-magnetic beads and binds ALFA-tagged proteins with extraordinarily high affinity (Kd = 26 pM). Target proteins are efficiently released from ALFA Catcher ST under acidic (e.g., 0.1 M glycin pH2.2) or denaturing conditions. The extremely tight interaction with ALFA-tagged proteins makes competitive elution using ALFA elution peptide inefficient. We recommend using ALFA Catcher PE for competitive elution under physiological and room temperature condition conditions. For efficient elutions at 4°C, we recommend using the ALFA Catcher CE

The innovative, oriented and highly selective attachment of the active sdAbs via a flexible linker guarantees optimal accessibility for target proteins and at the same time largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no “leakage” of light and heavy chains from IgGs is observed even during elution with strongly denaturing and reducing conditions (e.g., SDS sample buffer). ALFA Catcher ST thus features high affinity and superior capacity for ALFA fusion proteins while showing negligible non-specific background.

Ingrédients: 4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody

Matériel non inclus: wash buffers, columns, tubes

Information d'application

Indications d'application: Coating: sdAb anti-ALFA tag clone 1G5
Matrix: 4 % cross-linked agarose, bead size 50-150 μm
Capacity: > 3 µg ALFA-tagged GFP per µL of packed beads (= 2 μL of slurry)
Buffer Compatibility:

• Common buffer substances at pH 5 to 10
• 1 % Triton X-100, > 1% DDM, > 1 % CHAPS, 0.1 % SDS
• > 3 M NaCl, > 1 M MgSO4
• 6 M urea, > 2 M Guanidin-HCl
• > 100 mM DTT

Commentaires:

ALFA Catcher ST is compatible not only with physiological buffers but also with high stringency buffers. ALFA Catcher ST thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

Restrictions: For Research Use only

Stockage

Buffer: Resin is 50 % slurry in PBS containing 20 % Ethanol.

Stock: 4 °C

Stockage commentaire: Store at 4°C for up to 12 months. Do not freeze!

Date de péremption: 12 months

Détail du antigène

Antigène: ALFA-Tag

Classe de substances: Tag

Sujet: The ALFA-tag is a novel, highly versatile epitope tag (core sequence SRLEEELRRRLTE) that can be employed for a wide range of life science applications.