CCA1 anticorps
L’anticorps anti-CCA1 Polyclonal Lapin est utilisé pour la détection de CCA1 dans des échantillons de Arabidopsis thaliana. Il a été validé pour Co-IP et WB.
N° du produit ABIN4966136
435,69 €
Plus frais de livraison 40,00 € et TVA
50 μg
Destination:
France
Envoi sous 3 à 5 jours ouvrables
Aperçu rapide pour CCA1 anticorps (ABIN4966136)
Antigène
CCA1
(Circadian Clock Associated 1 (CCA1))
Reactivité
Arabidopsis thaliana
Hôte
Lapin
Clonalité
Polyclonal
Application
Co-Immunoprecipitation ( Co-IP), Western Blotting (WB)
-
-
Fonction
- Anti-CCA1 | Circadian clock associated 1
-
Réactivité croisée (Details)
-
Arabidopsis thaliana
Not reactive in: Oryza sativa, Solanum lycopersicum -
Purification
- Immunogen affinity purified serum
-
Immunogène
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCA1 protein sequence
-
-
-
Indications d'application
- 1: 500 (Co-IP), 1 : 500 (WB)
-
Commentaires
-
Important note about protein extractionTranscription factors are best isolated by freezing nucelar extract in liquid nitrogen after adding the extraction buffer, followed by thawing directly in 100°C heating block not in RT. When the samples were heated for 10 min, spinning of the extract for 5 min is needed to remove the cell debris. From the liquid extract measurement can be done and the rest, frozen in liquid Nitrogen, in aliquots. Frozen samples will not be thawed again to 100°C, but on RT and loaded as soon as they are liquid. Such precautions are necessary to take to be able to detect a transcription factor using antibodies.
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- For reconstitution add 50 μL of sterile water
-
Buffer
- Immunogen affinity purified serum in PBS pH 7.4.
-
Conseil sur la manipulation
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
-
Stock
- -20 °C
-
Stockage commentaire
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
-
-
- CCA1 (Circadian Clock Associated 1 (CCA1))
-
Sujet
- CCA1 (Circadian clocl associated 1) encodes a transcriptional repressor that performs overlapping functions with LHY in a regulatory feedback loop that is closely associated with the circadian oscillator of Arabidopsis. Binds to the evening element in the promoter of TOC1 and represses TOC1 transcription. CCA1 and LHY colocalize in the nucleus and form heterodimers in vivo. CCA1 and LHY function synergistically in regulating circadian rhythms of Arabidopsis.
-
Poids moléculaire
- 67 | 80 kDa
-
ID gène
- 819296
-
UniProt
- P92973
Antigène
-
Vous êtes ici:
