CCA1 anticorps
L’anticorps Lapin Polyclonal anti-CCA1 a été validé pour WB. Il convient pour détecter CCA1 dans des échantillons de Arabidopsis thaliana.
N° du produit ABIN4966136
Aperçu rapide pour CCA1 anticorps (ABIN4966136)
Antigène
CCA1
(Circadian Clock Associated 1 (CCA1))
Reactivité
Arabidopsis thaliana
Hôte
Lapin
Clonalité
Polyclonal
Application
Western Blotting (WB)
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Réactivité croisée (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity are currently known
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Homologie
- please inquire
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Immunogène
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCA1 protein sequence, UniProt:P92973, TAIR:AT2G46830
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Indications d'application
- 1 : 500 with most sensitive ECL reagent (WB)
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Commentaires
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Important note about protein extractionTranscription factors are best isolated by freezing nucelar extract in liquid nitrogen after adding the extraction buffer, followed by thawing directly in 100°C heating block not in RT. When the samples were heated for 10 min, spinning of the extract for 5 min is needed to remove the cell debris. From the liquid extract measurement can be done and the rest, frozen in liquid Nitrogen, in aliquots. Frozen samples will not be thawed again to 100°C, but on RT and loaded as soon as they are liquid. Such precautions are necessary to take to be able to detect a transcription factor using antibodies.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water.
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Stock
- -20 °C
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Stockage commentaire
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- CCA1 (Circadian Clock Associated 1 (CCA1))
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Sujet
- CCA1 (Circadian clocl associated 1) encodes a transcriptional repressor that performs overlapping functions with LHY in a regulatory feedback loop that is closely associated with the circadian oscillator of Arabidopsis. Binds to the evening element in the promoter of TOC1 and represses TOC1 transcription. CCA1 and LHY colocalize in the nucleus and form heterodimers in vivo. CCA1 and LHY function synergistically in regulating circadian rhythms of Arabidopsis.
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Poids moléculaire
- 67 | 80 kDa
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UniProt
- P92973
Antigène
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