CDJ1 anticorps (N-Term)
Cet anticorps anti-CDJ1 est un anticorps Lapin Polyclonal détectant CDJ1 dans IP et WB. Adapté pour Chlamydomonas reinhardtii. Ce Primary Antibody a été cité dans 2+ publications.
(2 références)
N° du produit ABIN334548
Aperçu rapide pour CDJ1 anticorps (N-Term) (ABIN334548)
Antigène
CDJ1
(Chloroplast DnaJ Protein (CDJ1))
Reactivité
Chlamydomonas reinhardtii
Hôte
Lapin
Clonalité
Polyclonal
Application
Immunoprecipitation (IP), Western Blotting (WB)
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Épitope
- N-Term
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Réactivité croisée (Details)
- Not reactive in: higher plants
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Homologie
- Ostreococcus sp.
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Attributs du produit
- Expected / apparent Molecular Weight of the Antigene: 40 / 40 kDa
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Purification
- serum
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Immunogène
- fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli
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Indications d'application
- 1. 3000 with standard ECL (WB)
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Commentaires
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western blot has been done on 10-50 µg load per well, immunoprecipitation has been done on 5-50 µl
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 100 μL of sterile water.
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Conseil sur la manipulation
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
Stock
- -20 °C
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Stockage commentaire
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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: "FER1 and FER2 encoding two ferritin complexes in Chlamydomonas reinhardtii chloroplasts are regulated by iron." dans: Genetics, Vol. 179, Issue 1, pp. 137-47, (2008) (PubMed).
: "The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C." dans: Plant physiology, Vol. 148, Issue 4, pp. 2070-82, (2008) (PubMed).
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: "FER1 and FER2 encoding two ferritin complexes in Chlamydomonas reinhardtii chloroplasts are regulated by iron." dans: Genetics, Vol. 179, Issue 1, pp. 137-47, (2008) (PubMed).
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- CDJ1 (Chloroplast DnaJ Protein (CDJ1))
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Autre désignation
- CDJ1
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Sujet
- CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.
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Poids moléculaire
- expected: 40 kDa, apparent: 40 kDa
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UniProt
- Q66YD3
Antigène
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