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Chmp1b2 Protein (AA 1-199) (Strep Tag)

Cette protéine Recombinant Chmp1b2 est exprimée dans Cell-free protein synthesis (CFPS).
N° du produit ABIN3137023
à partir de
15.754,29 €
Frais d'envoi 40,00 €, glace carbonique 20,00 € et TVA exclus
250 μg
Destination: France
Envoi sous 52 à 62 jours ouvrables

Aperçu rapide pour Chmp1b2 Protein (AA 1-199) (Strep Tag) (ABIN3137023)

Antigène

Chmp1b2 (Charged multivesicular body protein 1b-2 (Chmp1b2))

Type de proteíne

Recombinant

Origine

Souris

Source

Cell-free protein synthesis (CFPS)

Application

ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Pureté

approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Classe de qualité

custom-made
  • Attributs du protein

    AA 1-199

    Purification/Conjugué

    Cette Chmp1b2 protéine est marqué à la Strep Tag.

    Séquence

    MSNMEKHLFN LKFAAKELNR NAKKCDKEEK AEKAKIKKAI QKGNTEVARI HAENAIRQKN QAINFLRMSA RVDAVAARVQ TAVTMGKVTK SMAGVVKSMD ATLRSMNLEK ISALMDKFEH QFETLDVQTQ QMEDTMSSTT TLTTPQNQVD MLLQEMADEA GLDLNMELPQ GQTGSVGASV ASTEQDELSQ RLARLRDQV
    Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

    Attributs du produit

    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification

    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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  • Indications d'application

    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    Commentaires

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

    Conseil sur la manipulation

    Avoid repeated freeze-thaw cycles.

    Stock

    -80 °C

    Stockage commentaire

    Store at -80°C.

    Date de péremption

    12 months
  • Antigène

    Chmp1b2 (Charged multivesicular body protein 1b-2 (Chmp1b2))

    Autre désignation

    Chmp1b2

    Sujet

    Charged multivesicular body protein 1B2 (Chromatin-modifying protein 1b-2) (CHMP1b-2),FUNCTION: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B and SPAST to the midbody of dividing cells (By similarity). {ECO:0000250}.

    Poids moléculaire

    22.2 kDa

    UniProt

    Q9CQD4
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