CLEC4A2 Kit ELISA
Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Rat CLEC4A2.
N° du produit ABIN6951053
Aperçu rapide pour CLEC4A2 Kit ELISA (ABIN6951053)
Antigène
CLEC4A2
(C-Type Lectin Domain Family 4, Member a2 (CLEC4A2))
Reactivité
Rat
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Fonction
- Rat CLEC4A2 ELISA Kit.
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Analytical Method
- Quantitative
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
- For Research Use only
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Date de péremption
- 6 months
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Antigène
- CLEC4A2 (C-Type Lectin Domain Family 4, Member a2 (CLEC4A2))
Autre désignation
- CLEC4A2
ID gène
- 297584
UniProt
- Q5YIS1
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