D1-Nt anticorps
Cet anticorps Lapin Polyclonal détecte spécifiquement D1-Nt dans WB. Il présente une réactivité envers Arabidopsis, Cyanobacteria (Nostoc) et Nicotiana tabacum.
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N° du produit ABIN165361
Aperçu rapide pour D1-Nt anticorps (ABIN165361)
Antigène
D1-Nt (PsbA)
Reactivité
Arabidopsis, Cyanobacteria (Nostoc), Nicotiana tabacum
Hôte
Lapin
Clonalité
Polyclonal
Application
Western Blotting (WB)
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Specificité
- Higher plants. According to sequence homology antisera may reacts in Synechocystis only with PsbA2/3 but not with PsbA1. For analysis of cyanobacterial D1 proteins the dilution 1: 1.000 is recommended.
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Immunogène
- synthetic peptide against PsbA N- terminus (a. a. 1 – 17) from Arabidopsis thaliana PsbA protein.
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Isotype
- IgG
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Indications d'application
- Western blot (1 : 2.000 for ECL)
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C
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- D1-Nt (PsbA)
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Sujet
- D1 is one the major proteins of the reaction centre of photosystem II which carries the Q B -binding site. D1 reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane. D1 protein is synthesized in a precursor form containing a C-terminal extension of 16 amino acid residues in cyanobacteria and 8 – 9 - in eukaryotes. The precursor D1 is co-translationally inserted into the thylakoid membrane and is C-terminally cleaved by CtpA protease (Anbudurai et al., 1994, PNAS 16, 8082 – 8086). D1 protein from higher plants and green algae can be phosphorylated by STN8 kinase (Bonardi et al., 2005, Nature 437, 1179 – 1182, Vaionen et al., 2005, J Biol. Chem. 280, 33679 – 33686).
Antigène
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