Proteinase K (Tritirachium album limber)

Application Note: Proteinase K is specifically formulated for molecular cloning, nucleic acid research and protein/peptide structural analysis. One unit liberates one μmole of Folin positive amino acids, measured as tyrosine, at 37° C at  pH 7.5 using urea denatured hemoglobin as the substrate.

Synonyms: Protease K, Endopeptidase K

Background: Proteinase K is a broad-spectrum serine protease and is able to digest native keratin (hair), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer: None