Il existe 1 publication pour ce produit.
L’anticorps anti-EXOS Polyclonal Poulet est utilisé pour la détection de EXOS dans des échantillons de Pseudomonas aeruginosa. Il a été validé pour WB.
Aperçu rapide pour EXOS anticorps (cleaved) (ABIN93557)
Antigène
EXOS
(ExoS Gene Product (EXOS))
Reactivité
Pseudomonas aeruginosa
Hôte
Poulet
Clonalité
Polyclonal
Application
Western Blotting (WB)
Épitope
cleaved
Fonction
Anti-ExoS | Exoenzyme S
Réactivité croisée (Details)
Pseudomonas aeruginosa Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Homologie
Pseudomonas aeruginosa
Purification
Purified, total IgY
Immunogène
amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.
Isotype
IgY
Indications d'application
1 : 5000 (WB)
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
Stock
4 °C
Stockage commentaire
Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Shen, Tsay, Chia, Wu, Lee, Hu: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." dans: Investigative ophthalmology & visual science, (2012) (PubMed).
Antigène
EXOS
(ExoS Gene Product (EXOS))
Autre désignation
Exoenzyme S (ExoS)
Sujet
Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.