HSP18.5 anticorps
Cet anticorps anti-HSP18.5 est un anticorps Lapin Polyclonal détectant HSP18.5 dans WB. Adapté pour Arabidopsis thaliana.
N° du produit ABIN619791
Aperçu rapide pour HSP18.5 anticorps (ABIN619791)
Antigène
HSP18.5 (HSP18.5-C)
(18.5 kDa Class I Heat Shock Protein (HSP18.5-C))
Reactivité
Arabidopsis thaliana
Hôte
Lapin
Clonalité
Polyclonal
Application
Western Blotting (WB)
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Réactivité croisée (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity are currently known
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Homologie
- dicots including: Glycine max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, monocots including: Zea mays
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Attributs du produit
- Expected / apparent Molecular Weight of the Antigene: 18.5 kDa
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Purification
- serum
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Immunogène
- recombinant Arabidopsis thaliana Hsp18.5
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Indications d'application
- 1 : 1000 with standard ECL (WB)
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Commentaires
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hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38°C for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 200 μL of sterile water.
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Stock
- -20 °C
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Stockage commentaire
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- HSP18.5 (HSP18.5-C) (18.5 kDa Class I Heat Shock Protein (HSP18.5-C))
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Autre désignation
- HSP18.5
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Sujet
- HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family. Synonymes:AtHsp18.5, 18.5 kDa heat shock protein
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Poids moléculaire
- 18.5 kDa
Antigène
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