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Souris IgG2b Isotype Control

FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa unconjugated
N° du produit ABIN1043029

Aperçu rapide pour Souris IgG2b Isotype Control (ABIN1043029)

Antigène

IgG2b

Clonalité

  • 144
  • 3
  • 2
Monoclonal

Application

Flow Cytometry (FACS), Isotype Control (IsoC), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

  • 42
  • 16
  • 15
  • 14
  • 11
  • 7
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
MPC-11

Isotype

IgG2b kappa
  • Hôte

    • 131
    • 78
    • 2
    Souris

    Fonction

    Mouse IgG2b Isotype Control Purified

    Specificité

    This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.

    Aucune reactivité croisée

    Humain, Souris, Rat

    Purification

    Purified by protein-A affinity chromatography.

    Pureté

    > 95 % (by SDS-PAGE)

    Immunogène

    KLH-coupled trinitrophenol
  • Indications d'application

    Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Do not freeze.

    Stock

    4 °C

    Stockage commentaire

    Store at 2-8°C. Do not freeze.
  • Dewals, Vanderplasschen et al.: "Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid ..." dans: Veterinary research, Vol. 42, Issue 1, pp. 95, (2011) (PubMed).

    Liang, Zhou, Li, Wan: "Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells." dans: World journal of gastroenterology, Vol. 16, Issue 5, pp. 618-24, (2010) (PubMed).

    Smed-Sörensen, Moll, Cheng, Loré, Norlin, Perbeck, Moody, Spetz, Sandberg: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." dans: Blood, Vol. 111, Issue 10, pp. 5037-46, (2008) (PubMed).

    Im, Tapinos, Chae, Illarionov, Besra, DeVries, Modlin, Sieling, Rambukkana, Porcelli: "Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5226-35, (2006) (PubMed).

    Gupta, Wang, Vinson, Dziarski: "Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1." dans: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14012-20, (1999) (PubMed).

  • Antigène

    IgG2b

    Autre désignation

    IgG2b kappa

    Classe de substances

    Antibody
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