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Souris IgM Isotype Control

ELISA, WB, SDS IgM unconjugated
Rockland
N° du produit ABIN1045087
N° du produit (Fournisseur): 010-0107

Aperçu rapide pour Souris IgM Isotype Control (ABIN1045087)

Antigène

IgM

Application

ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Isotype

IgM
  • Hôte

    • 62
    • 49
    • 34
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Souris

    N° du produit (Fournisseur)

    010-0107

    Fournisseur

    Rockland

    Fonction

    Mouse IgM Whole Molecule

    Réactivité croisée (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and anti-Mouse IgM (μ chain specific).   No reaction was observed against anti-Mouse IgG F(c).  Some light chain cross-reactivity will occur with anti-Mouse IgG.

    Attributs du produit

    Mouse Immunoglobulin M

    Purification

    Mouse IgM was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. 

    Stérilité

    Sterile filtered
  • Indications d'application

    Application Note: Mouse IgM whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

    Western Blot Dilution: user optimized

    ELISA Dilution: user optimized

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.16 mg/mL

    Buffer

    Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

    Stabilizer: None

    Preservative: 0.1 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

    Date de péremption

    12 months
  • Antigène

    IgM

    Classe de substances

    Antibody

    Sujet

    Background: Mouse IgM, or mouse Immunoglobulin M, purified protein is a basic antibody that is produced by B cells. Mouse IgM is by far the physically largest antibody in the human circulatory system. Mouse IgM is the first antibody to appear in response to initial exposure to antigen. IgM is predominantly produced in the spleen. Mouse IgM is formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites.
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