Souris IgG1 isotype control (PE)
Aperçu rapide pour Souris IgG1 isotype control (PE) (ABIN1045089)
Antigène
Clonalité
Conjugué
Application
Clone
Isotype
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Hôte
- Souris
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N° du produit (Fournisseur)
- 010-0840
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Fournisseur
- Rockland
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Fonction
- Mouse IgG1 Isotype Control Phycoerythrin Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG1 and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. Phycoerythrin Conjugated Mouse IgG1 was proven by Dot Blot.
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Attributs du produit
- Phycoerythrin conjugated Mouse IgG1 subclass isotype control, Mouse IgG1 isotype control PE conjugation
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Purification
- This product was prepared from ascitic fluid by affinity chromatography.
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Mouse IgG1 isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 Biotin
Mouse IgG1 isotype control (Cy5)FACS, ELISA Monoclonal 15H6 IgG1 Cy5
Mouse IgG1 isotype control (PE)FACS, ELISA Monoclonal 15H6 IgG1 PE
Mouse IgG1 isotype control (APC)FACS, ELISA Monoclonal 15H6 IgG1 APC
Mouse IgG1 isotype control (SPRD)FACS, ELISA Monoclonal 15H6 IgG1 SPRD
Mouse IgG1 isotype control (APC-Cy7)FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy7
Mouse IgG1 isotype control (APC-Cy5.5)FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy5.5
Mouse IgG1 isotype control (PE-Cy7)FACS, ELISA Monoclonal 15H6 IgG1 PE-Cy7
Mouse IgG1 isotype control (FITC)FACS, ELISA Monoclonal 15H6 IgG1 FITC
Mouse IgG1 isotype control (FITC)FACS, IsoC, DFA, IFA VI-AP IgG1 FITC
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Indications d'application
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG1 isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
FLISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
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Date de péremption
- 12 months
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- IgG1
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Classe de substances
- Antibody
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Sujet
- Background: Isotype control Mouse IgG1 is important for Flow Cytometry. Mouse IgG1 control has no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
Antigène
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