Rat IgG1 isotype control (FITC)
Aperçu rapide pour Rat IgG1 isotype control (FITC) (ABIN5608798)
Antigène
Clonalité
Conjugué
Application
Clone
Isotype
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Hôte
- Rat
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N° du produit (Fournisseur)
- 012-002-040
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Fournisseur
- Rockland
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Fonction
- Rat IgG1 Isotype Control Fluorescein Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rat IgG, and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG2a, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Rat IgG1 confirmed by Dot Blot.
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Attributs du produit
- Rat Isotype Control, Rat IgG1 FITC isotype, RAT IgG1 subclass isotype control
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Purification
- RAT IgG1 isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G.
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Stérilité
- Sterile filtered
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Labeling Ratio
- 2-8
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Mouse IgG1 isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 Biotin
Mouse IgG1 isotype control (Cy5)FACS, ELISA Monoclonal 15H6 IgG1 Cy5
Mouse IgG1 isotype control (PE)FACS, ELISA Monoclonal 15H6 IgG1 PE
Mouse IgG1 isotype control (APC)FACS, ELISA Monoclonal 15H6 IgG1 APC
Mouse IgG1 isotype control (SPRD)FACS, ELISA Monoclonal 15H6 IgG1 SPRD
Mouse IgG1 isotype control (APC-Cy7)FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy7
Mouse IgG1 isotype control (APC-Cy5.5)FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy5.5
Mouse IgG1 isotype control (PE-Cy7)FACS, ELISA Monoclonal 15H6 IgG1 PE-Cy7
Mouse IgG1 isotype control (FITC)FACS, ELISA Monoclonal 15H6 IgG1 FITC
Mouse IgG1 isotype control (FITC)FACS, IsoC, DFA, IFA VI-AP IgG1 FITC
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Indications d'application
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: RAT IgG1 isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
FLISA Dilution: User Optimized
ELISA Dilution: 1:2000 - 1:20,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Optional[Buffer]: None Preservative: 0.01 % (w/v) Sodium Azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
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Date de péremption
- 12 months
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- IgG1
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Classe de substances
- Antibody
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Sujet
- Background: RAT IgG1 isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 2c and μ - IgM, light chain kappa and lambda.
Antigène
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