Aperçu rapide pour Rat IgG1 isotype control (PE) (ABIN5608799)
Antigène
Clonalité
Conjugué
Application
Clone
Isotype
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Hôte
- Rat
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N° du produit (Fournisseur)
- 012-008-040
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Fournisseur
- Rockland
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Fonction
- Rat IgG1 Isotype Control Phycoerythrin Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-phycoerythrin, anti-Rat IgG and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG2a, IgG2b, IgG3, IgM, and IgA. Phycoerythrin Conjugated Rat IgG1 confirmed by Dot Blot.
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Attributs du produit
- Rat Isotype Control, Rat IgG1 PE isotype, RAT IgG1 subclass isotype control
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Purification
- RAT IgG1 isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G.
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Labeling Ratio
- 3.7
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Mouse IgG1 isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa Biotin
Mouse IgG1 isotype control (FITC)FACS, ELISA Monoclonal 15H6 IgG1 FITC
Mouse IgG1 isotype control (FITC)FACS, IsoC, DFA, IFA VI-AP IgG1 FITC
Mouse IgG1 isotype control (PE)FACS, ELISA Monoclonal 15H6 IgG1 PE
Mouse IgG1 isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p), Func Monoclonal MOPC-21 IgG1 kappa unconjugated
Mouse IgG1 isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa unconjugated
Mouse IgG1 isotype control (APC)FACS, ELISA Monoclonal 15H6 IgG1 APC
Mouse IgG1 isotype control (SPRD)FACS, ELISA Monoclonal 15H6 IgG1 SPRD
Mouse IgG1 isotype control (Biotin)FACS, ELISA Monoclonal 15H6 IgG1 Biotin
Mouse IgG1 isotype control (FITC)FACS, IsoC Monoclonal MOPC-21 IgG1 kappa FITC
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Indications d'application
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: RAT IgG1 isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
FLISA Dilution: User Optimized
ELISA Dilution: 1:2000 - 1:20,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 0.5 mg/mL
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Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
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Date de péremption
- 12 months
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- IgG1
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Classe de substances
- Antibody
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Sujet
- Background: RAT IgG1 isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 2c and μ - IgM, light chain kappa and lambda.
Antigène
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