Human IgG isotype control (PE)
Aperçu rapide pour Human IgG isotype control (PE) (ABIN5633236)
Antigène
Conjugué
Application
Isotype
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Hôte
- Human
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N° du produit (Fournisseur)
- 009-008-002
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Fournisseur
- Rockland
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Réactivité croisée (Details)
- Human IgG whole molecule Phycoerythrin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Human IgG and anti-Human Serum.
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Purification
- Human IgG whole molecule Phycoerythrin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Labeling Ratio
- 1-2
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Hamster IgG isotype control
ELISA, WB, IHC, SDS IgG unconjugated
Rabbit IgG isotype controlELISA, WB, IHC, SDS IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Rabbit IgG isotype control (Atto 550)FACS, IHC Polyclonal IgG Atto 550
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
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Indications d'application
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Flow Cytometry Dilution: 1:500-1:2500
Immunohistochemistry Dilution: 1:500-1:2500
Application Note: Human IgG whole molecule Phycoerythrin conjugated can be utilized as a control reagent in Flow Cytometry, Immunohistochemistry, and Western Blotting. Researchers should determine optimal titers for applications that are not stated.
Western Blot Dilution: 1:1,000-1:5,000
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
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Date de péremption
- 12 months
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- IgG
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Classe de substances
- Antibody
Antigène
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