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Human IgG isotype control (PE)

FACS, WB, IHC IgG PE
N° du produit ABIN5633236
  • Antigène Tous les produits IgG
    IgG
    Hôte
    • 140
    • 138
    • 121
    • 89
    • 86
    • 69
    • 57
    • 56
    • 47
    • 44
    • 38
    • 37
    • 34
    • 33
    • 27
    • 21
    • 16
    • 15
    • 9
    • 9
    • 4
    • 2
    Human
    Conjugué
    PE
    Application
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)
    Réactivité croisée (Details)
    Human IgG whole molecule Phycoerythrin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Human IgG and anti-Human Serum.
    Purification
    Human IgG whole molecule Phycoerythrin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Isotype
    IgG
    Labeling Ratio
    1-2
  • Indications d'application

    Flow Cytometry Dilution: 1:500-1:2500

    Immunohistochemistry Dilution: 1:500-1:2500

    Application Note: Human IgG whole molecule Phycoerythrin conjugated can be utilized as a control reagent in Flow Cytometry, Immunohistochemistry, and Western Blotting. Researchers should determine optimal titers for applications that are not stated.

    Western Blot Dilution: 1:1,000-1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
    Date de péremption
    12 months
  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
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