Mouton IgG Isotype Control
Aperçu rapide pour Mouton IgG Isotype Control (ABIN5633250)
Antigène
Fragment
Application
Isotype
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Hôte
- Mouton
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N° du produit (Fournisseur)
- 013-0104-0002
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Fournisseur
- Rockland
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Fonction
- Sheep IgG F(ab')2 Fragment
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep IgG, anti-Sheep IgG F(ab')2 and anti-Sheep Serum. No reaction was observed against anti-Sheep IgG F(c) or anti-Pepsin.
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Attributs du produit
- Sheep IgG F(ab')2 Fragment
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Purification
- This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.
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Stérilité
- Sterile filtered
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Indications d'application
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Application Note: Sheep IgG F(ab')2 Fragment has been tested in SDS-Page and are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 2.1 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Date de péremption
- 12 months
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- IgG
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Classe de substances
- Antibody
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Sujet
- Background: Sheep IgG F(ab')2 Fragment is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . Sheep IgG F(ab')2 Molecules lack the Fc portion of Sheep IgG and therefore receptors that bind Sheep IgG Fc will not bind Sheep IgG F(ab')2 Molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
Antigène
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