Cheval IgG Isotype Control
Aperçu rapide pour Cheval IgG Isotype Control (ABIN964293)
Antigène
Fragment
Application
Isotype
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Hôte
- Cheval
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N° du produit (Fournisseur)
- 008-0104
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Fournisseur
- Rockland
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Fonction
- Horse IgG F(ab')2 Fragment
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse IgG, anti-Horse IgG F(ab')2 and anti-Horse Serum. No reaction was observed against anti-Horse IgG F(c) or anti-Pepsin.
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Attributs du produit
- Horse IgG F(ab')2 Fragment, Horse IgG Fab2 Fragment
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Purification
- This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.
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Stérilité
- Sterile filtered
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Indications d'application
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Application Note: Horse IgG F(ab')2 has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 2.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C upon receipt. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- IgG
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Classe de substances
- Antibody
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Sujet
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
Antigène
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