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Human IgG Isotype Control

Name: Human IgG Isotype Control
Brand: Rockland
SKU: ABIN964313
Availability: InStock

ELISA, WB, SDS IgG unconjugated F(ab')2 fragment

SDS-Page of Human IgG F(ab’)2 Fragment. (Human IgG Isotype Control)

SDS

1 image

N° du produit ABIN964313

N° du produit (Fournisseur): 009-0104

292,17 €

Plus frais de livraison 40,00 € et TVA

2 mg

Destination: France

Envoi sous 5 à 7 jours ouvrables

Aperçu rapide pour Human IgG Isotype Control (ABIN964313)

Antigène

IgG

Fragment

F(ab')2 fragment

Application

ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Isotype

IgG

Hôte
140

139

138

94

92

80

60

55

46

42

38

35

34

32

25

18

15

14

11

8

3

2
Human

N° du produit (Fournisseur)

009-0104

Fournisseur

Rockland

Fonction

Human IgG F(ab')2 Fragment

Réactivité croisée (Details)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(ab')2. No reaction was observed against anti-Human IgG F(c) or anti-Pepsin.

Attributs du produit

Human Immunoglobulin G F(ab')2 Fragment, IgG Fab2

Purification

Human IgG F(ab')2 was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.

Stérilité

Sterile filtered
Rabbit IgG isotype control
IsoC Polyclonal IgG unconjugated

Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Rabbit IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Rabbit IgG isotype control
FACS, IHC, IP Polyclonal IgG unconjugated

Rabbit IgG isotype control (Atto 550)
FACS, IHC Polyclonal IgG Atto 550

Mouse IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Mouse IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Goat IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Rabbit IgG isotype control (PE)
FACS, ELISA Polyclonal IgG PE

Indications d'application

Application Note: Human IgG F(ab')2 Fragment has been tested in SDS-Page and ELISA and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:5000 - 1:50,000
Other: User Optimized

Restrictions

For Research Use only
Format

Liquid

Concentration

2.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Date de péremption

12 months
Antigène

IgG

Classe de substances

Antibody

Sujet

Background: Human IgG F(ab')2 purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . Human IgG F(ab')2 Molecules lack the Fc portion of Human IgG and therefore receptors that bind Human IgG Fc will not bind Human IgG F(ab')2 Molecules.