Human IgG isotype control (HRP)
Aperçu rapide pour Human IgG isotype control (HRP) (ABIN964320)
Antigène
Fragment
Conjugué
Application
Isotype
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Hôte
- Human
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N° du produit (Fournisseur)
- 009-0304
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Fournisseur
- Rockland
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Fonction
- Human IgG F(ab')2 Fragment Peroxidase Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Human IgG, anti-Human IgG F(ab')2 and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Pepsin.
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Attributs du produit
- Human IgG F(ab')2 fragment peroxidase conjugation, Human IgG F(ab')2 fragment HRP conjugated
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Purification
- This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.
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ELISA, WB, IHC, SDS IgG unconjugated
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Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
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Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
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Indications d'application
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Application Note: Human IgG F(ab')2 fragment Peroxidase antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: None -
Agent conservateur
- Without preservative
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- IgG
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Classe de substances
- Antibody
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Sujet
- Background: Human IgG F(ab')2 purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . Human IgG F(ab')2 Molecules lack the Fc portion of Human IgG and therefore receptors that bind Human IgG Fc will not bind Human IgG F(ab')2 Molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
Antigène
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