Pour une meilleure utilisation, nous vous conseillons d’activer Javascript dans votre navigateur.

Human IgG isotype control (Biotin)

Name: Human IgG isotype control (Biotin)
Brand: Rockland
SKU: ABIN964324
Availability: InStock

ELISA, WB, IHC, DB IgG Biotin Fc fragment

Dot Blot of Human IgG F(c) Fragment Biotin Conjugated. (Human IgG isotype control (Biotin))

1 image

N° du produit ABIN964324

N° du produit (Fournisseur): 009-0603

402,65 €

Plus frais de livraison 40,00 € et TVA

1 mg

Destination: France

Envoi sous 5 à 7 jours ouvrables

Aperçu rapide pour Human IgG isotype control (Biotin) (ABIN964324)

Antigène

IgG

Fragment

Fc fragment

Conjugué

Biotin

Application

ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Isotype

IgG

Hôte
140

139

138

94

92

80

60

55

46

42

38

35

34

32

25

18

15

14

11

8

3

2
Human

N° du produit (Fournisseur)

009-0603

Fournisseur

Rockland

Fonction

Human IgG Fc Fragment Biotin Conjugated

Réactivité croisée (Details)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab')2 or anti-Papain.

Attributs du produit

Human Immunoglobulin G F(c) fragment, IgG Fc, Vitamin H, Coenzyme R

Purification

Human IgG F(c) fragment Biotin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
Rabbit IgG isotype control
IsoC Polyclonal IgG unconjugated

Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Rabbit IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Rabbit IgG isotype control
FACS, IHC, IP Polyclonal IgG unconjugated

Rabbit IgG isotype control (Atto 550)
FACS, IHC Polyclonal IgG Atto 550

Mouse IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Mouse IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Goat IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Rabbit IgG isotype control (PE)
FACS, ELISA Polyclonal IgG PE

Indications d'application

Immunohistochemistry Dilution: 1:500
Application Note: Human IgG F(c) fragment Biotin conjugated has been tested in dot blot and can be utilized as a control reagent in both Western Blotting and ELISA format experiments.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:5000 - 1:50,000
Other: User Optimized

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL

Concentration

1.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Human IgG F(c) fragment Biotin conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption

12 months
Antigène

IgG

Classe de substances

Antibody

Sujet

Background: Human IgG Fc purified protein is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of Human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.