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Souris IgG Isotype Control

Name: Souris IgG Isotype Control
SKU: ABIN964336
Price: 255.22 EUR
Availability: InStock

ELISA, WB, SDS, IHC IgG unconjugated Fab fragment

SDS-PAGE of Mouse IgG Fab Fragment . (Souris IgG Isotype Control)

SDS

Coommassie stained SDS-PAGE of 20 µl of Mouse Derived NIH 3T3 Whole Cell Lysate (Ready-to-Use) separated in a 4-20% gradient gel under non-reducing conditions (lane 1). (Souris IgG Isotype Control)

2 images

N° du produit ABIN964336

N° du produit (Fournisseur): 010-0105

Aperçu rapide pour Souris IgG Isotype Control (ABIN964336)

Antigène

IgG

Fragment

Fab fragment

Application

ELISA, Western Blotting (WB), SDS-PAGE (SDS), Immunohistochemistry (IHC)

Isotype

IgG

Hôte
137

127

123

84

82

81

55

52

46

42

38

33

33

32

25

18

10

8

8

7

3

2
Souris

N° du produit (Fournisseur)

010-0105

Fournisseur

Rockland

Fonction

Mouse IgG Fab Fragment

Réactivité croisée (Details)

Mouse IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG, anti-Mouse IgG F(ab')2 and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(c) or anti- Papain.

Attributs du produit

Mouse Immunoglobulin Fab, F(ab), Fragment antigen-binding

Purification

Mouse IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.

Stérilité

Sterile filtered
Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Rabbit IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated

Mouse IgG isotype control
FACS, ELISA, WB, IF (cc), IF (p), IHC (fro), IHC (p) Polyclonal IgG unconjugated

Mouse IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Rabbit IgG isotype control (PE)
FACS, ELISA Polyclonal IgG PE

Goat IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Rabbit IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Goat IgG isotype control
IsoC, ELISA, IHC Polyclonal IgG unconjugated

Mouse IgG isotype control
FACS, ELISA Polyclonal IgG unconjugated

Indications d'application

Immunohistochemistry Dilution: User Optimized
Application Note: Mouse IgG Fab Fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized

Restrictions

For Research Use only
Format

Liquid

Concentration

2.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Date de péremption

12 months
Antigène

IgG

Classe de substances

Antibody

Sujet

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.