Aperçu rapide pour Souris IgG isotype control (HRP) (ABIN964342)
Antigène
Fragment
Conjugué
Application
Isotype
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Hôte
- Souris
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N° du produit (Fournisseur)
- 010-0304
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Fournisseur
- Rockland
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Fonction
- Mouse IgG F(ab')2 Fragment Peroxidase Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Mouse IgG, anti-Mouse IgG F(ab')2 and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(c) or anti-Pepsin.
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Attributs du produit
- Peroxidase conjugated Mouse IgG F(ab')2 fragment, HRP conjugated Mouse IgG F(ab')2 fragment
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Purification
- This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.
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Hamster IgG isotype control
ELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlELISA, WB, SDS, IHC IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Rabbit IgG isotype control (Atto 550)FACS, IHC Polyclonal IgG Atto 550
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Goat IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype control (PE)FACS, ELISA Polyclonal IgG PE
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Indications d'application
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Application Note: Mouse IgG F(ab')2 fragment Peroxidase can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Buffer
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
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Agent conservateur
- Gentamicin sulfate
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Précaution d'utilisation
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- IgG
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Classe de substances
- Antibody
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Sujet
- Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region.
Antigène
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