Mouton IgG isotype control (Biotin)
Aperçu rapide pour Mouton IgG isotype control (Biotin) (ABIN964413)
Antigène
Fragment
Conjugué
Application
Isotype
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Hôte
- Mouton
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N° du produit (Fournisseur)
- 013-0604
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Fournisseur
- Rockland
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Fonction
- Sheep IgG F(ab')2 Fragment Biotin Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Sheep IgG, anti-Sheep IgG F(ab')2 and anti-Sheep Serum. No reaction was observed against anti-Sheep IgG F(c) or anti-Pepsin.
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Attributs du produit
- Sheep IgG F(ab')2 Fragment Biotin conjugate, Sheep IgG Fab2 Fragment Biotin conjugation
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Purification
- This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.
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Hamster IgG isotype control
ELISA, WB, IHC, SDS IgG unconjugated
Rabbit IgG isotype controlELISA, WB, IHC, SDS IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Rabbit IgG isotype control (Atto 550)FACS, IHC Polyclonal IgG Atto 550
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlIsoC, FACS, ELISA, IHC Polyclonal IgG unconjugated
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
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Indications d'application
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Application Note: Sheep IgG F(ab')2 Fragment Biotin Conjugated reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- IgG
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Classe de substances
- Antibody
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Sujet
- Background: Sheep IgG F(ab')2 Fragment Biotin Conjugated is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . Sheep IgG F(ab')2 Molecules lack the Fc portion of Sheep IgG and therefore receptors that bind Sheep IgG Fc will not bind Sheep IgG F(ab')2 Molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region followed by biotin conjugation.
Antigène
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