Pour une meilleure utilisation, nous vous conseillons d’activer Javascript dans votre navigateur.

Souris IgG1 Isotype Control

Name: Souris IgG1 Isotype Control
SKU: ABIN964460
Price: 245.28 EUR
Availability: InStock

FACS, ELISA, WB, FM, FLISA, SDS Monoclonal MG1L IgG1 unconjugated

SDS-PAGE of Mouse IgG1 Lambda Isotype Control. (Souris IgG1 Isotype Control)

SDS

1 image

(1 reference)

N° du produit ABIN964460

N° du produit (Fournisseur): 010-001-331

Aperçu rapide pour Souris IgG1 Isotype Control (ABIN964460)

Antigène

IgG1

Clonalité

Monoclonal

Application

Flow Cytometry (FACS), ELISA, Western Blotting (WB), Fluorescence Microscopy (FM), FLISA, SDS-PAGE (SDS)

Clone

MG1L

Isotype

IgG1

Hôte
221

59

28

6

4

2

2

1

1

1
Souris

N° du produit (Fournisseur)

010-001-331

Fournisseur

Rockland

Fonction

Mouse IgG1 Lambda (λ) Isotype Control

Réactivité croisée (Details)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.

Attributs du produit

Mouse immunoglobulin gamma lambda, Mouse IgG1 isotype control, Mouse IgG1 Lambda

Purification

Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.

Stérilité

Sterile filtered
Mouse IgG1 isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa Biotin

Mouse IgG1 isotype control (FITC)
FACS, ELISA Monoclonal 15H6 IgG1 FITC

Mouse IgG1 isotype control (PE)
FACS, ELISA Monoclonal 15H6 IgG1 PE

Mouse IgG1 isotype control
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p), Func Monoclonal MOPC-21 IgG1 kappa unconjugated

Mouse IgG1 isotype control
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa unconjugated

Mouse IgG1 isotype control (SPRD)
FACS, ELISA Monoclonal 15H6 IgG1 SPRD

Mouse IgG1 isotype control (APC)
FACS, ELISA Monoclonal 15H6 IgG1 APC

Mouse IgG1 isotype control (Biotin)
FACS, ELISA Monoclonal 15H6 IgG1 Biotin

Mouse IgG1 isotype control (PE-Cy7)
FACS, ELISA Monoclonal 15H6 IgG1 PE-Cy7

Mouse IgG1 isotype control (APC-Cy7)
FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy7

Indications d'application

Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG1 lambda isotype control has been tested in SDS-Page and ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized

Restrictions

For Research Use only
Format

Liquid

Concentration

1.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store vial at 4° C prior to opening. Mouse IgG1 Lambda isotype control is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Date de péremption

12 months
Klaile, Müller, Schäfer, Clauder, Feer, Heyl, Stock, Klassert, Zipfel, Singer, Slevogt: "Binding of Candida albicans to Human CEACAM1 and CEACAM6 Modulates the Inflammatory Response of Intestinal Epithelial Cells." dans: mBio, Vol. 8, Issue 2, (2017) (PubMed).
Antigène

IgG1

Classe de substances

Antibody

Sujet

Background: Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda. IgG1 is the most abundant of the four IgG subclasses. This isotype control possesses lambda light chains.