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Souris IgG3 Isotype Control

FACS, ELISA, WB, FLISA, FM, SDS Monoclonal MG3 IgG3 unconjugated
Rockland
N° du produit ABIN964479
N° du produit (Fournisseur): 010-0143

Aperçu rapide pour Souris IgG3 Isotype Control (ABIN964479)

Antigène

IgG3

Clonalité

  • 46
  • 2
  • 1
Monoclonal

Application

Flow Cytometry (FACS), ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), SDS-PAGE (SDS)

Clone

  • 18
  • 14
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
MG3

Isotype

IgG3
  • Hôte

    • 64
    • 6
    • 6
    • 1
    • 1
    Souris

    N° du produit (Fournisseur)

    010-0143

    Fournisseur

    Rockland

    Fonction

    Mouse IgG3 Isotype Control

    Réactivité croisée (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum.  Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Confirmed by SDS-PAGE.

    Attributs du produit

    MOUSE IgG3 subclass isotype control, MOUSE IgG3 isotype control

    Purification

    Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.

    Stérilité

    Sterile filtered
  • Indications d'application

    Flow Cytometry Dilution: 1:1000-1:5000

    Application Note: Mouse IgG3 isotype control has been tested by SDS-Page and ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

    Western Blot Dilution: User Optimized

    FLISA Dilution: User Optimized

    ELISA Dilution: User Optimized

    IF Microscopy Dilution: User Optimized

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Date de péremption

    12 months
  • Antigène

    IgG3

    Classe de substances

    Antibody

    Sujet

    Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
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