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Souris IgG3 isotype control (FITC)

FACS, ELISA, WB, FLISA, FM, DB Monoclonal MG3 IgG3 FITC
Rockland
N° du produit ABIN964480
N° du produit (Fournisseur): 010-0243

Aperçu rapide pour Souris IgG3 isotype control (FITC) (ABIN964480)

Antigène

IgG3

Clonalité

  • 38
  • 2
  • 1
Monoclonal

Conjugué

FITC

Application

Flow Cytometry (FACS), ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Clone

  • 18
  • 6
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
MG3

Isotype

IgG3
  • Hôte

    • 56
    • 6
    • 6
    • 1
    • 1
    Souris

    N° du produit (Fournisseur)

    010-0243

    Fournisseur

    Rockland

    Fonction

    Mouse IgG3 Isotype Control Fluorescein Conjugated

    Réactivité croisée (Details)

    Typically less than 1% cross reactivity against other mouse heavy or light chain specific antibodies was detected by ELISA.

    Attributs du produit

    Fluorescein conjugated Mouse IgG3 isotype control, Mouse IgG3 subclass isotype control FITC conjugation

    Purification

    This product has been prepared from serum-free tissue culture supernate by protein A chromatography. 

    Labeling Ratio

    3.3
  • Indications d'application

    Flow Cytometry Dilution: 1:1000-1:5000

    Application Note: Mouse IgG3 isotype has been tested in dot blot and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    Western Blot Dilution: User Optimized

    FLISA Dilution: User Optimized

    ELISA Dilution: User Optimized

    IF Microscopy Dilution: User Optimized

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Reconstitution Volume: 100 μL

    Concentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Date de péremption

    12 months
  • Antigène

    IgG3

    Classe de substances

    Antibody

    Sujet

    Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
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