Cat (Feline) IgM Isotype Control
Aperçu rapide pour Cat (Feline) IgM Isotype Control (ABIN964486)
Antigène
Application
Isotype
-
-
Hôte
- Cat (Feline)
-
N° du produit (Fournisseur)
- 002-0107
-
Fournisseur
- Rockland
-
Fonction
- Cat IgM Whole Molecule
-
Réactivité croisée (Details)
- Cat IgM whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Cat Serum and anti-Cat IgM (μ chain specific). No reaction was observed against anti-Cat IgG F(c). Some light chain cross reactivity will occur with anti-Cat IgG.
-
Attributs du produit
- Cat Immunoglobulin M
-
Purification
- Cat IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
-
Stérilité
- Sterile filtered
-
-
-
-
Indications d'application
-
Immunohistochemistry Dilution: User Optimized
Application Note: Cat IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 1.1 mg/mL
-
Buffer
- Optional[Buffer]: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 Preservative: 0.1 % (w/v) Sodium Azide
-
Agent conservateur
- Sodium azide
-
Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Stock
- 4 °C,-20 °C
-
Stockage commentaire
- Store vial at 4° C prior to opening. Cat IgM whole molecule is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
-
Date de péremption
- 12 months
-
-
- IgM
-
Classe de substances
- Antibody
-
Sujet
- Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
Antigène
-