Aperçu rapide pour Poulet IgM Isotype Control (ABIN964487)
Antigène
Application
Isotype
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Hôte
- Poulet
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N° du produit (Fournisseur)
- 003-0107
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Fournisseur
- Rockland
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Fonction
- Chicken IgM Whole Molecule
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Réactivité croisée (Details)
- Chicken IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and anti-Chicken IgM (μ chain specific). No reaction was observed against anti-Chicken IgG F(c). Some light chain cross reactivity will occur with anti-Chicken IgG. Analysis by SDS-PAGE was used to show purity at greater than 95%. Some high molecular weight banding may be visible. A minor band at ~50 kDa may also be visible.
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Attributs du produit
- Chicken Immunoglobulin M, Fowl IgM
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Purification
- Chicken IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
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Pureté
- >95 %
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Stérilité
- Sterile filtered
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Mouse IgM isotype control (FITC)
FACS, ELISA Monoclonal 11E10 IgM FITC
Rat IgM isotype controlFACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Mouse IgM isotype controlIsoC, FACS, ELISA, WB, IHC (p), IHC (fro), ICC, IP Monoclonal PFR-03 IgM unconjugated
Mouse IgM isotype control (PE)FACS, ELISA Monoclonal 11E10 IgM PE
Rabbit IgM isotype controlIsoC, ELISA, IHC Polyclonal IgM unconjugated
Mouse IgM isotype control (Biotin)FACS, ELISA Monoclonal 11E10 IgM Biotin
Rat IgM isotype control (PE)FACS, ELISA Monoclonal NIP-M-2 IgM PE
Mouse IgM isotype control (APC)FACS, ELISA Monoclonal 11E10 IgM APC
Mouse IgM isotype control (SPRD)FACS, ELISA Monoclonal 11E10 IgM SPRD
Mouse IgM isotype control (FITC)IsoC, FACS Monoclonal PFR-03 IgM FITC
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Indications d'application
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Immunohistochemistry Dilution: User Optimized
Application Note: Chicken IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
- Optional[Buffer]: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 Preservative: 0.1 % (w/v) Sodium Azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Chicken IgM whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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Date de péremption
- 12 months
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- IgM
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Classe de substances
- Antibody
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Sujet
- Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
Antigène
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