Human IgM isotype control (Rhodamine)
Aperçu rapide pour Human IgM isotype control (Rhodamine) (ABIN964501)
Antigène
Fragment
Conjugué
Application
Isotype
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Hôte
- Human
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N° du produit (Fournisseur)
- 009-0009
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Fournisseur
- Rockland
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Fonction
- Human IgM (myeloma) Fab μ Fragment Rhodamine Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human IgM and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Papain.
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Attributs du produit
- Human IgM Fab μ Rhodamine conjugation, Human myeloma IgM Fabμ TRITC conjugation
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Purification
- This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.
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Labeling Ratio
- 2.6
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Indications d'application
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Application Note: Human IgM Fab μ fragment Rhodamine conjugation is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- IgM
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Classe de substances
- Antibody
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Sujet
- Background: Human IgM Fab mu Rhodamine is ideal for investigators involved in serum protein component research. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen. The spleen is the major site of specific IgM production. Distinct heavy chains differ in size and composition, alpha and gamma contain approximately 450 amino acids, while μ and ε have approximately 550 amino acids.
Antigène
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