Human IgM isotype control (FITC)
Aperçu rapide pour Human IgM isotype control (FITC) (ABIN964507)
Antigène
Fragment
Conjugué
Application
Isotype
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Hôte
- Human
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N° du produit (Fournisseur)
- 009-0231
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Fournisseur
- Rockland
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Fonction
- Human IgM (myeloma) Fc5μ Fragment Fluorescein Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human IgM Fc5μ and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab')2 or anti-Trypsin.
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Attributs du produit
- Human IgM Fc5μ fluorescein conjugation, Human myeloma IgM Fc5μ FITC conjugation
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Purification
- This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by trypsin digestion and extensive dialysis against the buffer stated above.
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Labeling Ratio
- 2.0
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Indications d'application
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Application Note: Human IgM (myeloma) Fc5μ fragment Fluorescein conjugation is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- IgM
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Classe de substances
- Antibody
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Sujet
- Background: Human IgM (myeloma) Fc5μ fragment Fluorescein conjugate consists of only the μ (mu) chain of the Fc fragment. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. IgM is formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum.
Antigène
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