Human IgM isotype control (Biotin)
Aperçu rapide pour Human IgM isotype control (Biotin) (ABIN964511)
Antigène
Conjugué
Application
Isotype
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Hôte
- Human
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N° du produit (Fournisseur)
- 009-0607
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Fournisseur
- Rockland
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Fonction
- Human IgM (myeloma) Whole Molecule Biotin Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgM and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Pepsin.
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Attributs du produit
- Human IgM whole molecule Biotin conjugated, human myeloma IgM whole molecule biotin conjugation
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Purification
- This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation, ion exchange chromatography followed by tandem molecular sieve chromatography and extensive dialysis against the buffer stated above.
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Mouse IgM isotype control (FITC)
FACS, ELISA Monoclonal 11E10 IgM FITC
Rat IgM isotype controlFACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Mouse IgM isotype controlIsoC, FACS, ELISA, WB, IHC (fro), IHC (p), ICC, IP Monoclonal PFR-03 IgM unconjugated
Mouse IgM isotype control (PE)FACS, ELISA Monoclonal 11E10 IgM PE
Rabbit IgM isotype controlIsoC, ELISA, IHC Polyclonal IgM unconjugated
Mouse IgM isotype control (Biotin)FACS, ELISA Monoclonal 11E10 IgM Biotin
Rat IgM isotype control (PE)FACS, ELISA Monoclonal NIP-M-2 IgM PE
Mouse IgM isotype control (APC)FACS, ELISA Monoclonal 11E10 IgM APC
Mouse IgM isotype control (SPRD)FACS, ELISA Monoclonal 11E10 IgM SPRD
Mouse IgM isotype control (FITC)IsoC, FACS Monoclonal PFR-03 IgM FITC
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Indications d'application
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Application Note: Human IgM (myeloma) Biotin conjugated whole molecule can be used as a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- IgM
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Classe de substances
- Antibody
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Sujet
- Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
Antigène
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