Souris IgM Isotype Control
Aperçu rapide pour Souris IgM Isotype Control (ABIN964516)
Antigène
Clonalité
Application
Clone
Isotype
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Hôte
- Souris
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N° du produit (Fournisseur)
- 010-001-339
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Fournisseur
- Rockland
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Fonction
- Mouse IgM Kappa (k) Isotype Control
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, Ig3, IgA. Light chain composition has been confirmed by SDS-PAGE.
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Attributs du produit
- Mouse immunoglobulin M kappa, Mouse IgM isotype control, mouse IgM kappa
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Purification
- Mouse IgM Kappa isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
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Stérilité
- Sterile filtered
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Mouse IgM isotype control (FITC)
FACS, ELISA Monoclonal 11E10 IgM FITC
Rat IgM isotype controlFACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Mouse IgM isotype controlIsoC, FACS, ELISA, WB, IHC (p), IHC (fro), ICC, IP Monoclonal PFR-03 IgM unconjugated
Mouse IgM isotype control (PE)FACS, ELISA Monoclonal 11E10 IgM PE
Rabbit IgM isotype controlIsoC, ELISA, IHC Polyclonal IgM unconjugated
Mouse IgM isotype control (Biotin)FACS, ELISA Monoclonal 11E10 IgM Biotin
Rat IgM isotype control (PE)FACS, ELISA Monoclonal NIP-M-2 IgM PE
Mouse IgM isotype control (APC)FACS, ELISA Monoclonal 11E10 IgM APC
Mouse IgM isotype control (SPRD)FACS, ELISA Monoclonal 11E10 IgM SPRD
Mouse IgM isotype control (FITC)IsoC, FACS Monoclonal PFR-03 IgM FITC
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Indications d'application
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgM kappa isotype control has been tested in ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
- Optional[Buffer]: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 Preservative: 0.1 % (w/v) Sodium Azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to opening. Mouse IgM Kappa (κ) isotype control is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Date de péremption
- 12 months
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- IgM
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Classe de substances
- Antibody
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Sujet
- Background: Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda.
Antigène
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