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Souris IgM Isotype Control

Name: Souris IgM Isotype Control
SKU: ABIN964517
Price: 245.28 EUR
Availability: InStock

FACS, ELISA, WB, SDS, FM, FLISA Monoclonal MML IgM unconjugated

SDS-PAGE of Mouse IgM lambda isotype control. (Souris IgM Isotype Control)

SDS

SDS-PAGE of Mouse IgM Lambda (λ) isotype control . (Souris IgM Isotype Control)

SDS

2 images

N° du produit ABIN964517

N° du produit (Fournisseur): 010-001-338

Aperçu rapide pour Souris IgM Isotype Control (ABIN964517)

Antigène

IgM

Clonalité

Monoclonal

Application

Flow Cytometry (FACS), ELISA, Western Blotting (WB), SDS-PAGE (SDS), Fluorescence Microscopy (FM), FLISA

Clone

MML

Isotype

IgM

Hôte
62

49

34

5

4

2

2

2

2

2

2

2

2

2

2

1

1
Souris

N° du produit (Fournisseur)

010-001-338

Fournisseur

Rockland

Fonction

Mouse IgM Lambda (λ) Isotype Control

Réactivité croisée (Details)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, Ig3, IgA. Light chain composition has been confirmed by SDS-PAGE.

Attributs du produit

Mouse IgM Lambda isotype Control, Mouse IgM Lambda subclass isotype Control

Purification

Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.

Stérilité

Sterile filtered
Mouse IgM isotype control (FITC)
FACS, ELISA Monoclonal 11E10 IgM FITC

Rat IgM isotype control
FACS, ELISA Monoclonal NIP-M-2 IgM unconjugated

Mouse IgM isotype control (PE)
FACS, ELISA Monoclonal 11E10 IgM PE

Mouse IgM isotype control
FACS, IsoC, ELISA, WB, IHC (fro), IHC (p), ICC, IP Monoclonal PFR-03 IgM unconjugated

Rabbit IgM isotype control
IsoC, ELISA, IHC Polyclonal IgM unconjugated

Rat IgM isotype control (PE)
FACS, ELISA Monoclonal NIP-M-2 IgM PE

Mouse IgM isotype control (APC)
FACS, ELISA Monoclonal 11E10 IgM APC

Mouse IgM isotype control (Biotin)
FACS, ELISA Monoclonal 11E10 IgM Biotin

Mouse IgM isotype control (SPRD)
FACS, ELISA Monoclonal 11E10 IgM SPRD

Mouse IgM isotype control (PE-Cy7)
FACS, ELISA Monoclonal 11E10 IgM PE-Cy7

Indications d'application

Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgM lambda isotype control has been tested in SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized

Restrictions

For Research Use only
Format

Liquid

Concentration

1.0 mg/mL

Buffer

Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Stabilizer: None
Preservative: 0.1 % (w/v) Sodium Azide

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Date de péremption

12 months
Antigène

IgM

Classe de substances

Antibody

Sujet

Background: Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda.