Formaldehyde Assay Kit

N° du produit ABIN1000253

Détail du produit

Antigène: Formaldehyde

Application: Biochemical Assay (BCA)

Type d'échantillon: Urine

Specificité: 1.5 μM

Attributs du produit: Safe. Non-radioactive assay.
Sensitive and accurate. As low as 1.5 µM Formaldehyde can be quantified.
Homogeneous and convenient. Mix-incubate-measure type assay. No wash and reagent transfer steps are involved.
Robust and amenable to HTS: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Ingrédients: Reagent A: 5 mL. Reagent B: 3 mL. Standard: 50 µL. 10% TCA: 5 mL. Neutralizer: 2 x 1.5 mL.

Matériel non inclus: Pipetting devices, centrifuge tubes, black flat bottom 96-well plates (e.g. Corning Costar) and plate reader.

Information d'application

Indications d'application: Formaldehyde determination in urine and other biological samples.

Protocole: Use black flat-bottom plates. Prior to assay, bring all reagents to room temperature.
1. Standards. First prepare a 35 mM Formaldehyde stock by diluting 4 µL of the provided Standard with 1490 µL dH2O. Next, dilute 4 µL of the 35 mM Formaldehyde with 1396 µL dH2O to make a 100 µM Premix. Transfer 50 µL standards into separate wells of the plate.
2. Add 50 µL of each prepared sample to two separate wells of the plate (one well will be used as a Sample Blank).
3. Prepare Working Reagent for each standard and sample well by mixing 33 µL Reagent A and 22 µL Reagent B. For the Sample Blanks, make the following Working Reagent: 33 µL Reagent A + 22 µL dH2O. Add 50 µL of the appropriate Working Reagent to each well. Tap plate to mix. Incubate at room temperature for 30 min protected from light.
5. Read fluorescence intensity at exc = 370 nm and em = 470 nm.

Préparation de l'échantillon:

Urine samples should be diluted 2-5 fold with dH2O. If urine samples contain visible particulates, then the samples should be cleared by either filtration or centrifugation (14000 rpm, 5 min). Samples high in protein (cell lysate, serum, etc.) need to be deproteinated and neutralized prior to assaying. To deproteinate, add 50 µL 10% TCA per 100 µL sample. Vortex and centrifuge for 5 min at 14000 rpm. Transfer 100 µL of clear supernatant to a clean tube and neutralize with 25 µL Neutralizer. Note: Measured RFU's for deproteinated samples need to be multiplied by
1.875 to compensate for the resulting dilution of the sample. Samples not measured the same day should be stored frozen, preferably at -80°C.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Formaldehyde

Sujet: Quantitative determination of formaldehyde concentration by fluorimetric (370nm/470nm) method.

Formaldehyde (methanal) is the simplest aldehyde. It is widely employed in industry for wide range of applications. Formaldehyde is also used as a disinfectant and is a commonly utilized tissue fixative and embalming agent. Formaldehyde is naturally present in all tissues and body fluids. Recently it has been shown that some cancer types exhibit elevated formaldehyde levels. Increased formaldehyde concentration in urine has been associated with prostate and bladder cancer. Thus, measuring formaldehyde in urine can be a very useful tool when studying cancer. This newly designed Formaldehyde Assay Kit provides a convenient fluorimetric means to measure formaldehyde in biological samples. In the assay, formaldehyde is derivatized with acetoacetanilide in the presence of ammonia. The resulting fluorescent product is then quantified fluorimetrically (exc/em = 370/470nm). The assay is simple, sensitive, stable and high-throughput adaptable. The assay can detect as low as 1.5 µM formaldehyde in biological samples.