Neuraminidase Assay Kit

N° du produit ABIN1000322

Aperçu rapide pour Neuraminidase Assay Kit (ABIN1000322)

Antigène: NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))

Application: Activity Assay (AcA)

Détail du produit

Specificité: 0.1 - 10 U/L (colorimetric), 0.01 - 2 U/L (fluorimetric)

Attributs du produit: Sensitive and accurate. Linear detection range at 37°C in 96-well plate: 0.1 to 10 U/L for colorimetric assays and 0.01 to 2 U/L for fluorimetric assays.
Simple and convenient.
Homogeneous assay requiring only two absorbance measurements. Assay can be completed in 60 min.
High-throughput. Can be readily automated as a high-throughput 96- well plate assay to screen thousands of samples per day.

Ingrédients: Assay Buffer: 6 mL. Substrate: 6 mL. Cofactors: 120 µL. Dye Reagent: 60 µL. Enzyme: 120 µL. Standard: 500 µL.

Matériel non inclus: Pipetting devices, centrifuge tubes, Clear flat-bottom 96-well plates, black 96-well or 384-well plates (e.g. Corning Costar) and plate reader.

Information d'application

Indications d'application: Direct Assays: neuraminidase activity in biological samples.
Drug Discovery: evaluation of neuraminidase inhibitors.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Référence

Li, Ma, Sarker, McClane: "CodY is a global regulator of virulence-associated properties for Clostridium perfringens type D strain CN3718." dans: mBio, Vol. 4, Issue 5, pp. e00770-13, (2013) (PubMed).

Détail du antigène

Antigène: NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))

Autre désignation: Neuraminidase

Classe de substances: Influenza Protein

Sujet: Quantitative determination of neuraminidase activity or screening for neuraminidase inhibitor by colorimetric (570nm) or fluorimetric (530nm/590nm) methods.
Procedure: 60 min.

Neuramindase (also known as Sialidase) is an enzyme that hydrolyzes terminal sialic acid residues on poly-saccharide chains. It is predominantly expressed in microorganisms such as bacteria and viruses. Cleavage of sialic acid residues by neuraminidase is believed to play several roles in infection by influenza viruses. It is thought to assist in the penetration of mucosal linings, the invasion of target cells, the elution of progeny viruses from infected cells, and the prevention of self-aggregation. Thus, neuraminidase is an important target for influenza drug development and simple, direct and automation-ready procedures for measuring neuraminidase activity find wide applications in research and drug discovery. This neuraminidase assay measures the sialic acid released by neuraminidase in one step. The change in color intensity of the reaction product at 570nm or fluorescence intensity at gamma em/ex = 585/530nm is directly proportional to neuraminidase activity in the sample.