BMP2 Kit ELISA

N° du produit ABIN1112564

Détail du produit BMP2 Kit ELISA

Antigène: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: For quantitative detection of BMP-2 in serum, plasma, body fluids, tissue lysates or cell culture supernates.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Sensibilité: < 2 pg/mL

Ingrédients: 1. One 96-well plate pre-coated with anti-Mouse BMP-2 antibody 2. Lyophilized BMP-2 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse BMP-2 antibody (Concentrated): 130 µl.

Matériel non inclus: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Information d'application

Commentaires:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- BMP-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- BMP-2 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the BMP-2 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of BMP-2 can be calculated.

Plaque: Pre-coated

Préparation des réactifs:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Préparation de l'échantillon:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Tissue lysate or body fluids, cell culture supernate: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 10 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with EDTA as the anticoagulant. Centrifuge for 10min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate or heparin can not be used as anticoagulant here. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Détail du antigène

Antigène: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Autre désignation: BMP-2 (BMP2 Produits)

Synonymes: BDA2 Kit ELISA, BMP2A Kit ELISA, AI467020 Kit ELISA, Bmp2a Kit ELISA, BMP-2 Kit ELISA, xBMP-2 Kit ELISA, xbmp2 Kit ELISA, BMP2 Kit ELISA, bmp2a Kit ELISA, bmp2 Kit ELISA, wu:fc59d09 Kit ELISA, bone morphogenetic protein 2 Kit ELISA, bone morphogenetic protein 2 L homeolog Kit ELISA, Bone morphogenetic protein 2 Kit ELISA, bone morphogenetic protein 2a Kit ELISA, BMP2 Kit ELISA, Bmp2 Kit ELISA, bmp2.L Kit ELISA, bmp2 Kit ELISA, bmp2a Kit ELISA

Sujet: Bone morphogenetic protein 2 (BMP-2) belongs to the TGF-beat superfamily of proteins. Like other family members, it plays an important role in the development of bone and cartilage. It has been demonstrated to potently induce osteoblast differentiation in a variety of cell types.BMP-2 has an ability to induce the formation of ectopic cartilage when implanted subcutaneously.

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation