MMP1 Kit ELISA

N° du produit ABIN1112753

Détail du produit MMP1 Kit ELISA

Antigène: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Analytical Method: Quantitative

Sensibilité: < 10 pg/mL

Ingrédients: 1. One 96-well plate pre-coated with anti-Human MMP-1 antibody 2. Lyophilized Human MMP-1 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-human MMP-1 antibody (Concentrated): 130 µl.

Matériel non inclus: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Information d'application

Commentaires:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MMP-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP-1 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the MMP-1 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of MMP-1 can be calculated.

Plaque: Pre-coated

Préparation des réactifs:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Préparation de l'échantillon:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Tissue lysate, body fluids and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin as the anticoagulant. Centrifuge for 15 min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate and EDTA can not be used as anticoagulant here. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Détail du antigène

Antigène: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Autre désignation: MMP-1 (MMP1 Produits)

Synonymes: CLG Kit ELISA, CLGN Kit ELISA, Mmp1a Kit ELISA, CG4859 Kit ELISA, Dm1-MMP Kit ELISA, Dmel\\CG4859 Kit ELISA, MMP-1 Kit ELISA, MMP1 Kit ELISA, Mmp 1 Kit ELISA, dMMP1 Kit ELISA, dm1-MMP Kit ELISA, dmmp1 Kit ELISA, l(2)k04809 Kit ELISA, mmp1 Kit ELISA, Mmp1 Kit ELISA, Mcol-A Kit ELISA, Mcola Kit ELISA, col4 Kit ELISA, mmp18 Kit ELISA, Clgn Kit ELISA, matrix metallopeptidase 1 Kit ELISA, Matrix metalloproteinase 1 Kit ELISA, matrix metalloproteinase 1 Kit ELISA, matrix metallopeptidase 1 (interstitial collagenase) Kit ELISA, matrix metallopeptidase 1a (interstitial collagenase) Kit ELISA, matrix metallopeptidase 1 S homeolog Kit ELISA, interstitial collagenase Kit ELISA, matrix metallopeptidase 8 L homeolog Kit ELISA, MMP1 Kit ELISA, Mmp1 Kit ELISA, RB11133 Kit ELISA, Mmp1a Kit ELISA, mmp1.S Kit ELISA, LOC100727966 Kit ELISA, mmp8.L Kit ELISA

Sujet: Matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase and fibroblast collagenase , is a member of the matrix metalloproteinases family. Matrix metalloproteinases are zinc-dependent proteases that degrade extracellular matrix proteins. MMP-1 is structurally formed by different proteic building blocks. The Catalytic Domain of MMP-1 is composed of five highly twisted beta-strands (sI-sV), three Į-helix (hA-hC) and a total of eight loops, enclosing a total of five metal ions, three Ca2+ and two Zn2+, one of which with catalytic role. MMP1 in the stromal-tumor microenvironment can alter the behavior of cancer cells through PAR1 to promote cell migration and invasion. The biologic implications of MMP1 acting as a molecular ratchet tethered to the cell surface suggested novel mechanisms for its role in tissue remodeling and cell-matrix interaction.