MMP3 Kit ELISA

N° du produit ABIN1112757

Le kit ELISA MMP3 (ABIN1112757) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain MMP3.

Aperçu rapide pour MMP3 Kit ELISA (ABIN1112757)

Antigène: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum, Tissue Lysate

Détail du produit MMP3 Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: For quantitative detection of MMP-3 in Human serum, plasma, tissue lysatesor cell culture supernatants.

Analytical Method: Quantitative

Sensibilité: < 30 pg/mL

Ingrédients: 1. One 96-well plate pre-coated with anti-Human MMP-3 antibody 2. Lyophilized Human MMP-3 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-human MMP-3 antibody (Concentrated): 130 µl.

Matériel non inclus:

1. 37 °C incubator
2. Microplate reader (wavelength: 450 nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5 mL of Eppendorf tubes
7. Plate cover
8. Absorbent filter papers
9. Plastic or glass container with volume of above 1 L

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MMP-3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP-3 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the MMP-3 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of MMP-3 can be calculated.

Plaque: Pre-coated

Préparation des réactifs:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37 °C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Préparation de l'échantillon:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Tissue lysate, body fluids and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin as the anticoagulant. Centrifuge for 15 min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate and EDTA can not be used as anticoagulant here. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 2-8°C for 6 months, or at -20°C for 12 months.

Date de péremption: 12 months

Détail du antigène

Antigène: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Autre désignation: MMP-3

Sujet: Matrix metalloproteinase-3 (MMP-3) also known as Stromelysin-1 is an enzyme that in humans is encoded by the MMP3 gene. This gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. It is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. The MMP-3 enzyme degrades collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP-3 can also activate other MMPs such as MMP-1, MMP-3, and MMP-9, rendering MMP-3 crucial in connective tissue remodeling. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation.