Resistin Kit ELISA

N° du produit ABIN1112787

Le kit ELISA Resistin (ABIN1112787) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris Resistin.

Aperçu rapide pour Resistin Kit ELISA (ABIN1112787)

Antigène: Resistin (RETN)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum, Tissue Lysate

Détail du produit Resistin Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: For quantitative detection of Resist in in mouse serum, plasma, tissue lysates,cell culture supernatants.

Analytical Method: Quantitative

Sensibilité: < 3 pg/mL

Ingrédients: 1. One 96-well plate pre-coated with anti-mouse Resistin antibody 2. Lyophilized Mouse Resistin standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse Resistin antibody (Concentrated): 130 µl.

Matériel non inclus:

1. 37 °C incubator
2. Microplate reader (wavelength: 450 nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5 mL of Eppendorf tubes
7. Plate cover
8. Absorbent filter papers
9. Plastic or glass container with volume of above 1 L

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Resistin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Resistin polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the Resistin amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of Resistin can be calculated.

Plaque: Pre-coated

Préparation des réactifs:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37 °C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Préparation de l'échantillon:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Body fluids, tissue lysates and cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin or EDTA as the anticoagulant. Centrifuge for 30 min at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate can not be used as anticoagulant here. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 2-8°C for 6 months, or at -20°C for 12 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Resistin (RETN)

Autre désignation: Resistin

Sujet: Resistin also known as adipose tissue-specific secretory factor or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1) is a cysteine-rich protein that in humans is encoded by the RETN gene. The gene comprises 4 exons, the first of which is untranslated, and spans approximately 1,750 bp. The length of the resistin pre-peptide in human is 108 amino acid residues and in the mouse and rat it is 114 aa, the molecular weight is ~12.5 kDa. Resistin is a cytokine whose physiologic role has been the subject of much controversy regarding its involvement with obesity and type II diabetes mellitus (T2DM).

Pathways: Feeding Behaviour, Smooth Muscle Cell Migration