IgG Kit ELISA
Aperçu rapide pour IgG Kit ELISA (ABIN1113184)
Antigène
Voir toutes IgG Kits ELISAÉpitope
Reactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 16 ng/mL
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Fonction
- Rapid quantification of IgG in cell culture supernatant. Screening of higher producing clones, follow-up of production process, Pharmacokinetic studies
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Marque
- FastELISA®
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Analytical Method
- Quantitative
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Specificité
- The method enables the detection of natural human IgG as well as recombinant humanized or fully human monoclonal antibodies. Chimeric (human-mouse) recombinant antibodies are detected with the method
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Réactivité croisée (Details)
- < 1% for the following IgG: Mouse, Cow, Horse, Sheep and Swine and are < 2 % for Rabbit IgG.
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Sensibilité
- 4 ng/mL
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Attributs du produit
- The human immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of human IgG in cell culture supernatant and serum.
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IgG ELISA Kit
Reactivité: Lapin Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Rabbit TrueBlot® Set (with IP beads)WB, IP Reactivité: Lapin
Mouse TrueBlot® Set (with IP beads)WB, IP Reactivité: Souris
IgG ELISA KitReactivité: Souris Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivité: Humain Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivité: Poulet Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivité: Souris Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivité: Rat Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivité: Porc Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
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Commentaires
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One Step ELISA 30 min total time
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Durée du test
- < 1 h
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Plaque
- Pre-coated
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Protocole
- A polyclonal antibody specific to the gamma heavy chain of human IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and human IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-human IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of human IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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Préparation des réactifs
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dilution
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Date de péremption
- 12 months
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- IgG
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Autre désignation
- Immunoglobulin G
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Classe de substances
- Antibody
Antigène Voir toutes IgG Kits ELISA
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