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Immunoglobulin isotyping

IsoT Reactivité: Souris Colorimetric Cell Culture Supernatant, Purified Protein Pre-coated
N° du produit ABIN1113186
  • Antigène Voir toutes Ig Kits
    Ig
    Épitope
    Heavy & Light Chain
    Reactivité
    • 4
    • 4
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Conjugué
    HRP
    Application
    Isotyping (IsoT)
    Fonction
    The method employs the quantitative sandwich enzyme immunoassay technique.
    Marque
    FastELISA®
    Type d'échantillon
    Cell Culture Supernatant, Purified Protein
    Specificité
    Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, Kappa chain,
    Attributs du produit
    The mouse immunoglobulin isotyping kit provides a rapid and easy method (one step ELISA) to characterize mouse monoclonal antibody isotypes in cell culture supernatants or purified antibodies preparations.
    Top Product
    Discover our top product Ig Kit ELISA
  • Commentaires

    One Step ELISA 30 min total time

    Durée du test
    < 1 h
    Plaque
    Pre-coated
    Protocole
    Anti-mouse antibodies specific to each of the common light and heavy chains are pre-coated in the wells. Samples are pipetted into microwells and Ig present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops if the specific immunoglobulin is present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
    Préparation des réactifs

    dilution

    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène
    Ig
    Autre désignation
    Immunoglobulins (Ig Produits)
    Synonymes
    ATPIG Kit, ATPIQ Kit, A330005H02Rik Kit, AI315324 Kit, Ig Kit, ATPase phospholipid transporting 11C Kit, ATPase, class VI, type 11C Kit, ATP11C Kit, Atp11c Kit
    Sujet
    Isotyping of mouse immunoglobulins
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