IgG Kit ELISA

N° du produit ABIN1113190

Kit ELISA Boeuf (Vache) IgG, test Colorimetric pour la quantification de Boeuf (Vache) IgG.

Aperçu rapide pour IgG Kit ELISA (ABIN1113190)

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Boeuf (Vache)

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 10-2000 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum

Détail du produit IgG Kit ELISA

Seuil minimal de détection: 10 ng/mL

Fonction: Rapid quantification of bovine IgG in batches of purified antibodies, selection of low IgG sera

Marque: FastELISA®

Analytical Method: Quantitative

Specificité: The method enables the detection of bovine IgG.

Attributs du produit: The bovine immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of bovine IgG in cell culture supernatants and serums.

Information d'application

Commentaires:

One Step ELISA 45 min total time
IgG contaminations of about 20 ppm

Durée du test: < 1 h

Plaque: Pre-coated

Protocole: A polyclonal antibody specific to bovine IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and bovine IgG present in the sample are bound by the capture antibody. Then, an HRP (horseradish peroxidase) conjugated anti-bovine IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of bovine IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Préparation des réactifs:

dilution

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Autre désignation: Immunoglobulin G

Classe de substances: Antibody