TOLLIP Kit ELISA
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- TOLLIP (Toll Interacting Protein (TOLLIP))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 31.2-2000 pg/mL
- Seuil minimal de détection
- 31.2 pg/mL
- Application
- ELISA
- Fonction
- This immunoassay kit allows for the in vitro quantitative determination of human Toll-interacting protein, concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
- Analytical Method
- Quantitative
- Specificité
- This assay recognizes recombinant and natural human. No significant cross-reactivity or interference was observed.
- Ingrédients
- Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)
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- Commentaires
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Gene Name: TOLLIP
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Stock
- 4 °C/-20 °C
- Stockage commentaire
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Antigène Voir toutes TOLLIP Kits ELISA
- TOLLIP (Toll Interacting Protein (TOLLIP))
- Autre désignation
- Toll-interacting protein (TOLLIP Produits)
- Synonymes
- IL-1RAcPIP Kit ELISA, 4930403G24Rik Kit ELISA, 4931428G15Rik Kit ELISA, fk22a02 Kit ELISA, im:7145391 Kit ELISA, wu:fk22a02 Kit ELISA, zgc:76985 Kit ELISA, TOLLIP Kit ELISA, GB17961 Kit ELISA, tollip Kit ELISA, tollip1 Kit ELISA, tollipa Kit ELISA, TOLIP Kit ELISA, toll interacting protein Kit ELISA, toll-interacting protein Kit ELISA, toll-interacting protein-like Kit ELISA, toll interacting protein L homeolog Kit ELISA, toll interacting protein S homeolog Kit ELISA, Toll-interleukine I receptor interacting protein I Kit ELISA, TOLLIP Kit ELISA, Tollip Kit ELISA, tollip Kit ELISA, LOC552034 Kit ELISA, LOC100169858 Kit ELISA, tollip.L Kit ELISA, tollip.S Kit ELISA, LOC100136122 Kit ELISA
- Sujet
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Synonyms: Homo sapiens,Human,Toll-interacting protein,TOLLIP
Subunit: Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2.
Function: Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. - UniProt
- Q9H0E2
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