Fecal Calprotectin Rapid Test Kit

N° du produit ABIN1305183

Détail du produit

Antigène: Calprotectin (S100A8/A9)

Reactivité: Humain

Application: Rapid Test (rapid)

Fonction: The Epituub Calprotectin test is a ?sandwich? immunoassay utilizing two monoclonal antibodies to specifically detect the presence of Calprotectin in feces. It consists of two units, a fecal sampling device and a test strip. A stool specimen is collected into the sampling tube containing extraction solution. After mixing the stool sample, a test strip is screwed into the sampling tube by breaking the bottom seal of the sampling tube while maintaining a vertical position. The extracted fecal solution flows into the bottom space of the test strip and triggers the start of the Calprotectin immunoassay. If the Calprotectin level is greater than 50 ?g/g in a fecal sample extract, an immuno-complex of ?labeled monoclonal anticalprotectin antibody ? membrane coated monoclonal anti-human calprotectin antibody? is formed. A red colored band appears in the test region, which is located in the lower half of the test membrane. A similar colored band must appear in the control region located in the upper half of the test membrane, indicating the test strip is functioning properly and the result is valid.

Marque: EpiTuub®

Type d'échantillon: Fecal

Analytical Method: Qualitative

Réactivité croisée (Details): this Calprotectin rapid test does not cross-react to human albumin or hemoglobin.

Ingrédients: 1. Fecal specimen collection device (30436): contains sampling tube, sampling lid and pre-added extraction solution in the sampling tube. This device should be stored at 2 to 8 C. Do not freeze. Test strip tube (30437): one dipstick for the Calprotectin test is assembled in a transparent housing and sealed in a foil pouch with desiccant. It should remain in its original sealed pouch until ready for use. The test strip should be stored at 2 to 30 C. Do not freeze.
3. Instruction for use.

Matériel non inclus: 1. Timer or clock

Information d'application

Commentaires:

Single pouched.

Durée du test: 0.5 h

Protocole: This Epituub Calprotectin Test Device is a rapid immunological test intended for the qualitative detection of Calprotectin in feces by professional laboratories and physician office laboratories. The test is used as an aid in the diagnosis of inflammatory bowel disease. Fecal Calprotectin levels correlate significantly with histological and endoscopic assessment of disease activity in ulcerative collitis, as well as with fecal - antitrypsin levels and fecal excretion of indium labeled white blood cells in patients with Crohn's disease.

Prélèvement de l'échantillon: Stool specimens can be collected at any time of the day.
2. Collect a random sample of feces in a clean, dry cup or toilet paper or as indicated in the Figure 1.
3. Unscrew the sampling lid and keep the sampling tube in a vertical position to prevent the loss of any extraction solution.
4. Insert and twist the tip of the sampling lid into the stool specimen at two or more different sites (Figure 2).
5. Collect fecal sample that is stuck to the surface of the sampling lid. The total amount of stool sample should be less than one grain of cooked rice. Do not intentionally collect any separate and large pieces of fecal sample into the tube.
6. Replace the sampling lid into the tube and secure tightly (Figure 3).
7. The specimen is ready for testing, transportation or storage. It can be stored at 2-8 °C for up to 21 days and at room temperature for up to 14 days.

Procédure de l'essai:

Bring the sealed foil pouch test strips and collected specimens to room temperature. 2. Shake the sampling tube vigorously to ensure a good liquid suspension. 3. Position the sampling tube upside down vertically and let it settle for about 1 minute. 4. Remove the test strip from the sealed foil pouch. 5. Screw the test strip tube into the sampling tube by breaking the bottom seal of the sampling tube. Secure tightly! (Figure A) 6. Allow the solution to flow into the bottom space of the test strip and keeping the device in a vertical position. 7. Read test result at 5 minutes. Do not interpret test result after 10 minutes.

Calcul des résultats:

1. Positive: If two red/pink colored bands are visible within 5 minutes, the test result is positive and valid (Figure B).
   2. Negative: If test area has no red/pink colored band and the control area displays a red/pink colored band, the test result is negative (Figure B). Refer to Limitation of the Procedure #4 for additional information.
   3. Invalid: If a colored band does not form in the control area regardless of there being any band in the test area, the test result is invalid (Figure B) and needs to be retested.

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: 1. For in-vitro diagnostic use only. Not to be taken internally. 2. Do not use product beyond the expiration date. 3. Handle all specimens as potentially infectious. 4. Do not reuse the test.

Stock: 4 °C

Détail du antigène

Antigène: Calprotectin (S100A8/A9)

Autre désignation: Calprotectin (S100A8/A9 Produits)

Synonymes: 60B8AG Kit, CAGB Kit, CFAG Kit, CGLB Kit, L1AG Kit, LIAG Kit, MAC387 Kit, MIF Kit, MRP14 Kit, NIF Kit, P14 Kit, Mrp14 Kit, MRP-14 Kit, p14 Kit, 60B8Ag Kit, AW546964 Kit, BEE22 Kit, Cagb Kit, GAGB Kit, L1Ag Kit, S100 calcium binding protein A9 Kit, S100 calcium binding protein A9 (calgranulin B) Kit, S100A9 Kit, S100a9 Kit

Sujet: Calprotectin (MRP 8/14) is a heterodimer of two calcium-binding proteins present in the cytoplasm of neutrophils and expressed by the membranes of monocytes. It constitutes nearly 60 % of the soluble cytosol proteins in neutrophils and plays a central role in neutrophils defense. Upon neutrophil activation or endothelial adhesion of monocytes, Calprotectin is released and may be detected in serum, body fluids or stool as a potentially useful clinical inflammatory marker.