Aperçu rapide pour ANA Screen IgG Kit ELISA (ABIN1326795)
Antigène
ANA Screen IgG
Reactivité
Humain
Méthode de détection
Colorimetric
Type de méthode
Competition ELISA
Application
ELISA
Type d'échantillon
Serum
Fonction
Diluted patient serum is added to wells coated with purified nuclear antigens. ANA IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.
Analytical Method
Qualitative
Plaque
Pre-coated
Restrictions
For Research Use only
Stock
4 °C
Antigène
ANA Screen IgG
Classe de substances
Antibody
Sujet
Antinuclear antibodies (ANA) are frequently present in patients with systemic lupus erythematosus (SLE) and, less commonly, in other autoimmune diseases Rheumatoid arthritis, Collagen vascular diseases, chronic liver diseases and systemic sclerosis (scleroderma). ANA bind to several nuclear antigens including DsDNA, SSDNA, RNP, Sm, SSA and SSB. ANA frequency increases with age in apparently healthy people, especially women after the age of 45 years. ANA ELISA is widely used as a screening procedure for different autoimmune diseases.