RNP/Sm IgG Kit ELISA

N° du produit ABIN1326887

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain RNP/Sm IgG. Il a été cité dans 1 publication.

Aperçu rapide pour RNP/Sm IgG Kit ELISA (ABIN1326887)

Antigène: RNP/Sm IgG

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Application: ELISA

Type d'échantillon: Serum

Détail du produit

Fonction: Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.

Analytical Method: Qualitative

Information d'application

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Référence

Rupasree, Naushad, Rajasekhar, Uma, Kutala: "Association of TLR4 (D299G, T399I), TLR9 -1486T>C, TIRAP S180L and TNF-? promoter (-1031, -863, -857) polymorphisms with risk for systemic lupus erythematosus among South Indians." dans: Lupus, Vol. 24, Issue 1, pp. 50-7, (2014) (PubMed).

Détail du antigène

Antigène: RNP/Sm IgG

Classe de substances: Antibody

Sujet: Systemic autoimmune disease is characterized by the presence of circulating auto-antibodies directed to a wide variety of cellular antigens. Systemic lupus erythematosis (SLE), commonly referred to as Lupus is the best known of these diseases. Other possible connective tissue diseases include mixed connective tissue disease (MCTD), Sjogren syndrome, sclerodema, and polymyositisdermatomyositis. The majority can be diagnosed by clinical presentation and their antibody profiles to the various antigens involved, which include dsDNA, Sm, RNP, Ro, La Scl-70, Jo1 and Histones. Therefore, immunoassays for autoantibodies are useful for diagnostic and prognostic evaluations of autoimmune disease. RNP Abs target U1 RNA 70kD, A or C proteins and are present in most MCTD patients and in about 15% of SLE patients. The Sm or Smith Abs target B'/B or D proteins and are present in approximately 30% of patients with SLE . Sm is a very specific marker for SLE. Sm antibodies are very rare in other autoimmune diseases and normals.