Sm IgG Kit ELISA
Reactivité: Humain
Colorimetric
Competition ELISA
Serum
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- Antigène
- Sm IgG
- Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Competition ELISA
- Application
- ELISA
- Fonction
- Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.
- Type d'échantillon
- Serum
- Analytical Method
- Qualitative
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- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- 4 °C
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- Antigène
- Sm IgG
- Classe de substances
- Antibody
- Sujet
- Systemic autoimmune disease is characterized by the presence of circulating auto-antibodies directed to a wide variety of cellular antigens.Systemic lupus erythematosis (SLE), commonly referred to as Lupus is the best known of these diseases.Other possible connective tissue diseases include mixed connective tissue disease (MCTD), Sjogren syndrome, sclerodema, and polymyositisdermatomyositis.The majority can be diagnosed by clinical presentation and their antibody profiles to the various antigens involved, which include dsDNA, SM, RNP, Ro, La Scl-70, Jo1 and Histones. Therefore, immunoassays for autoantibodies are useful for diagnostic and prognostic evaluations of autoimmune disease. The SM or Smith antigen is composed of nuclear RNA and several polypeptides.Antibodies to SM are present in approximately 30% of patients with SLE (systemic lupus erythematosis).SM is a very specific marker for SLE.SM antibodies are very rare in other autoimmune diseases and normals.
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